pJK148 (ATCC® 86956)

Applications: contains sequenceintegrating vectorshuttle vectorvector permitting positive selection for integrationvector permitting visual detection of recombinants  /  Depositors: JB Keeney

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Designations pJK148
Depositors JB Keeney
Biosafety Level 1
Vector Information
Size (kb): 5.3439998626708980
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pJK142
Intact vector size: 3.272
Type of vector: plasmid
Vector end: NdeI
Vector end: NdeI
Cloning sites: SacI SacII SpeI XbaI NotI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI SalI HincII XhoI ApaI DraII KpnI
Polylinker sites: SacI SacII SpeI XbaI NotI BamHI SmaI PstI EcoRI EcoRV
HindIII ClaI SalI HincII XhoI ApaI DraII KpnI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lac
marker(s): ampR
promoter: lac
replicon: pMB1
Cross references:
Vector: pJK148 (plasmid)
Promoters: Promoter lac
Construction: pJK142
Marker(s):ampR,leu1
Construct size (kb): 5.343999862670898
Features: insert detection: lacZ'
marker(s): ampR
marker(s): leu1+
promoter: lac
replicon: pMB1
Applications
contains sequence
integrating vector
shuttle vector
vector permitting positive selection for integration
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.2; PstI--5.2; HindIII--2.9, 2.4.
Shuttle vector for integrating sequences at leu1. Permits visual detection of recombinants by lacZalpha complementation.
Constructed from pJK142 (ATCC 86955) by inserting the leu1 gene at the NdeI site.
The leu1 gene contains the following unique restriction sites: StyI EcoNI Eco47III BsmI Bsu36I SplI Tth3I NdeI NruI SnaBI.
The order of the major feature in the plasmid is: ampR - pMB1 ori - 5' lacZ' - SacI/MCS/KpnI - 3' lacZ' - leu1.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
References

Keeney JB, Boeke JD. Efficient targeted integration at leu1-32 and ura4-294 in Schizosaccharomyces pombe. Genetics 136: 849-856, 1994. PubMed: 8005439

Jill B Keeney, personal communication

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Shipped dried
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
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