|
Permits
|
These permits may be required for shipping this product:
-
Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
|
|
Vector Information
|
Size (kb): 8.9000005722045900 Vector: pRB382 (plasmid) Promoters: Promoter vegII (B. subtilis) Construction: pUB110, pBR322, lacZ, vegII Marker(s):ampR,bleR,neoR Construct size (kb): 8.90000057220459 Features: marker(s): ampR
marker(s): bleR
marker(s): neoR
promoter: vegII (B. subtilis)
replicon: pMB1
replicon: pUB110
reporter group: 'lacZ
MCS: HindIII...BamHI
terminator: To phage lambda
transcription terminator: rrnB
|
|
Applications
|
expression vector shuttle vector vector permitting visual detection of activity in colonies
|
|
Comments
|
Restriction digests of the clone give the following sizes (kb): BamHI--9.5; EcoRI/BglI--5.1, 2.2, 1.1, 0.8; BglI/BglII--3.5, 2.2, 2.0, 1.4. The Bacillus subtilis promoter vegII initiates transcription in both B. subtilis and E. coli. Structural stability of the plasmid in B. subtilis can be affected by high levels of protein production. Under these conditions, cell growth and stability may be improved by reducing the antibiotic concentration in the media. May not be suitable for cloning very strong expression signals. Translational fusion shuttle vector using 'lacZ as reporter. Also contains the vegII promoter. Useful for construction of fusion proteins. Neo confers resistance to neomycin and kanamycin and ble confers resistance to bleomycin and phleomycin. The 5' truncated 'lacZ gene, lacking transcriptional and translational initiation signals, is useful in both E. coli and in B. subtilis, since the endogenous beta-galactosidase level in B. subtilis is low.
|
|
References
|
Bruckner R. A series of shuttle vectors for Bacillus subtilis and Eschericia coli. Gene 122: 187-192, 1992. PubMed: 1452028
|