pJA72 (ATCC® 77259)

Applications: YC-type (centromeric) shuttle vectorshuttle vectorvector for other usesvector permitting visual detection of recombinants  /  Depositors: SJ Elledge

Permits and Restrictions

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Designations pJA72
Depositors SJ Elledge
Biosafety Level 1
Vector Information
Size (kb): 4.8969998359680180
Vector: pJA72 (plasmid)
Promoters: Promoter lac
Construction: pUC, Tn903
Marker(s):TRP1,kanR
Construct size (kb): 4.896999835968018
Features: insert detection: lacZ'
marker(s): kanR, TRP1
promoter: lac
replicon: pMB1, ARSH4
centromere: CEN6
Applications
YC-type (centromeric) shuttle vector
shuttle vector
vector for other uses
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): PstI--2.8, 1.9; XbaI--3.0, 1.7; HindIII--2.7, 2.1; EcoRV--2.8, 1.9.
YC-type shuttle vector that can be used with the Tn3-based transposition system developed in Heffron's laboratory. Permits visual detection of recombinants by the lacZalpha complementation system.
The order of the major features in this plasmid is: lacZ'/MCS - CEN6 - ARS H4 - TRP1 - kanR - pMB1 ori.
Media Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Stephen J Elledge, personal communication

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component of:ATCC 77261
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