pJA72 (ATCC® 77259)

Applications: YC-type (centromeric) shuttle vectorshuttle vectorvector for other usesvector permitting visual detection of recombinants  /  Depositors: SJ Elledge

Permits and Restrictions

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Designations pJA72
Depositors SJ Elledge
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.8969998359680180
Vector: pJA72 (plasmid)
Promoters: Promoter lac
Construction: pUC, Tn903
Marker(s):TRP1,kanR
Construct size (kb): 4.896999835968018
Features: insert detection: lacZ'
marker(s): kanR, TRP1
promoter: lac
replicon: pMB1, ARSH4
centromere: CEN6
Applications
YC-type (centromeric) shuttle vector
shuttle vector
vector for other uses
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): PstI--2.8, 1.9; XbaI--3.0, 1.7; HindIII--2.7, 2.1; EcoRV--2.8, 1.9.
YC-type shuttle vector that can be used with the Tn3-based transposition system developed in Heffron's laboratory. Permits visual detection of recombinants by the lacZalpha complementation system.
The order of the major features in this plasmid is: lacZ'/MCS - CEN6 - ARS H4 - TRP1 - kanR - pMB1 ori.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Stephen J Elledge, personal communication

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component of:ATCC 77261
Shipped freeze-dried
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