pJA70 (ATCC® 77258)

Applications: YC-type (centromeric) shuttle vectorshuttle vectorvector for other usesvector permitting visual detection of recombinants  /  Depositors: SJ Elledge

Permits and Restrictions

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Designations pJA70
Depositors SJ Elledge
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.0780000686645510
DESCRIPTION OF VECTOR:
Intact vector size: 5.078
Type of vector: plasmid
Cloning sites: SacI SacII NotI XbaI SpeI BamHI SmaI EcoRI EcoRV ClaI SalI
XhoI ApaI
Polylinker sites: SacI BstXI SacII EagI NotI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII ClaI SalI XhoI ApaI KpnI
Construction: pUC, Tn903
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
centromere: CEN6
insert detection: lacZ'
marker(s): kanR, HIS3
promoter: lac
replicon: pMB1, ARSH4
Vector: pJA70 (plasmid)
Promoters: Promoter lac
Construction: pUC, Tn903
Marker(s):kanR,HIS3
Construct size (kb): 5.078000068664551
Features: insert detection: lacZ'
marker(s): kanR, HIS3
promoter: lac
replicon: pMB1, ARSH4
centromere: CEN6
Applications
YC-type (centromeric) shuttle vector
shuttle vector
vector for other uses
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): PstI--2.2, 1.8, 1.2; HindIII--3.1, 1.9, 0.2; KpnI--2.9, 2.1; EcoRI--5.1.
YC-type shuttle vector that can be used with the Tn3-based transposition system developed in Heffron's laboratory. Permits visual detection of recombinants by the lacZalpha complementation system.
The order of the major features in this plasmid is: lacZ'/MCS - CEN6 - ARS H4 - HIS3 - kanR - pMB1 ori.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Stephen J Elledge, personal communication

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component of:ATCC 77261
Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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