pJA70 (ATCC® 77258)

Applications: YC-type (centromeric) shuttle vectorshuttle vectorvector for other usesvector permitting visual detection of recombinants  /  Depositors: SJ Elledge

Permits and Restrictions

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Designations pJA70
Depositors SJ Elledge
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101 (ATCC 33694)
Vector Information
Size (kb): 5.0780000686645510
DESCRIPTION OF VECTOR:
Intact vector size: 5.078
Type of vector: plasmid
Cloning sites: SacI SacII NotI XbaI SpeI BamHI SmaI EcoRI EcoRV ClaI SalI
XhoI ApaI
Polylinker sites: SacI BstXI SacII EagI NotI XbaI SpeI BamHI SmaI PstI EcoRI
EcoRV HindIII ClaI SalI XhoI ApaI KpnI
Construction: pUC, Tn903
Host range: Saccharomyces cerevisiaeCandida robusta; Escherichia coli
Features (with orientation and position when available):
centromere: CEN6
insert detection: lacZ'
marker(s): kanR, HIS3
promoter: lac
replicon: pMB1, ARSH4
Vector: pJA70 (plasmid)
Promoters: Promoter lac
Construction: pUC, Tn903
Marker(s):kanR,HIS3
Construct size (kb): 5.078000068664551
Features: insert detection: lacZ'
marker(s): kanR, HIS3
promoter: lac
replicon: pMB1, ARSH4
centromere: CEN6
Applications
YC-type (centromeric) shuttle vector
shuttle vector
vector for other uses
vector permitting visual detection of recombinants
Comments
Restriction digests of the clone give the following sizes (kb): PstI--2.2, 1.8, 1.2; HindIII--3.1, 1.9, 0.2; KpnI--2.9, 2.1; EcoRI--5.1.
YC-type shuttle vector that can be used with the Tn3-based transposition system developed in Heffron's laboratory. Permits visual detection of recombinants by the lacZalpha complementation system.
The order of the major features in this plasmid is: lacZ'/MCS - CEN6 - ARS H4 - HIS3 - kanR - pMB1 ori.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Stephen J Elledge, personal communication

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component of:ATCC 77261
Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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