St14-1 (ATCC® 61372)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: J Mandel

Permits and Restrictions

View Permits

Designations St14-1
Species Homo sapiens, human
Depositors J Mandel
Vector
Construct size (kb): 7.400000095367432
Insert
DNA: genomic
Insert lengths(kb): 3.0
Gene product: DNA Segment, single copy [DXS52]
Alleles: B1, F1, F2, B2
Insert Size (kb) 3.0
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.4; EcoRI--4.4, 3.0; HindIII--4.4, 3.0; PstI--5.0, 0.96, 0.36, 0.24; HincII--4.0, 3.4.
To resolve some of the TaqI alleles with similar fragment sizes, the depositor suggests using a 0.9% agarose gel and allowing bromphenol blue migration of 15 cm.
The St14 sequence family appears to be dispersed over a 570 - 600 kb region of Xq28.
The TaqI B2 allele (4.1 + 1.4) is almost exclusively associated with the 3.4 kb allele from the VNTR polymorphism.
This probe also detects polymorphisms with MspI, DdeI, BglI, and Tth111I.
St14-1 detects genomic fragments of the following approximate sizes (kb): BamHI--3.6; HindIII--10.0; TaqI--3.9; EcoRI--10.0.
References

Oberle I, et al. The telomeric region of the human X chromosome long arm: presence of a highly polymorphic DNA marker and analysis of recombination frequency. Proc. Natl. Acad. Sci. USA 82: 2824-2828, 1985. PubMed: 2986139

Mandel JL, et al. Genetic mapping of the human X chromosome: linkage analysis of the q26-q28 region that includes the fragile X locus and isolation of expressed sequences. Cold Spring Harbor Symp. Quant. Biol. 51: 195-203, 1986. PubMed: 3472716

Oberle I, et al. Multipoint genetic mapping of the Xq26-q28 region in families with fragile X mental retardation and in normal families reveals tight linkage of markers in q26-q27. Hum. Genet. 77: 60-65, 1987. PubMed: 3502701

Vincent A, et al. The polymorphic marker DXS304 is within 5 centimorgans of the fragile X locus. Genomics 5: 797-801, 1989. PubMed: 2574147

Feil R, et al. Physical and genetic mapping of polymorphic loci in Xq28 (DXS15, DXS52, DXS134): analysis of a cosmid clone and a yeast artificial chromosome. Am. J. Hum. Genet. 46: 720-728, 1990. PubMed: 1969226

Barjon P, Schwartz C. New TaqI RFLPs at the DXS52 (St14) locus in the black population. Nucleic Acids Res. 17: 2149, 1989. PubMed: 2564665

Suthers GK, et al. Physical mapping of new DNA probes near the fragile X mutation (FRAXA) by using a panel of cell lines. Am. J. Hum. Genet. 47: 187-195, 1990. PubMed: 2378346

Jean Louis Mandel, personal communication

Product Sheet
Product Sheet
Product Sheet