pSM6 (ATCC® 53352)

Applications: expression vector  /  Depositors: G Grandi

Permits and Restrictions

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Designations pSM6
Depositors G Grandi
Biosafety Level 1
Host
Distribution host: Escherichia coli HB101
Vector Information
Size (kb): 8.0000000000000000
Vector: pSM6 (plasmid)
Promoters: Promoter ErmC
Construction: pE194, pBR322
Marker(s):tetR
Construct size (kb): 8.0
Features: marker(s): tetR
promoter: ErmC
replicon: pMB1, pE194
Applications
expression vector
Comments
pSM6 can be maintained in both E. coli and B. subtilis but can be used as a cloning vector only in E. coli because the tetracycline resistance is poorly expressed in B. subtilis.
Constructed by ligating pBR322 and pE194 after linearizing with PstI. The EcoRI site was destroyed and a HpaI/SstI fragment containing ErmC was removed. The ErmC promoter and ribosome binding sequence was inserted upstream of an EcoRI linker.
The EcoRI cloning site is 4 bp downstream of the ribosome binding sequence.
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C
References

Grandi G, et al. New plasmid expression vectors for Bacillus subtilis. Plasmid 16: 1-14, 1986. PubMed: 3090575

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Product Sheet
Product Sheet
Product Sheet