Escherichia coli (Migula) Castellani and Chalmers (ATCC® 47059)

Strain Designations: BNN132  /  Type Strain: no  /  Biosafety Level: 1

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Classification Enterobacteriaceae, Escherichia
Strain Designations BNN132
Application
transformation host
Type Strain no
Biosafety Level 1
Product Format frozen
Shipping Information Distributed: freeze-dried
Preceptrol® no
Genotype endA1 gyr96 hsdR17 supE44 relA1 delta(lac-proAB) (F' traD36 proA+ proB+ lacIq delta(lacZ)M15) lambdaKC(kan-cre)
Type Strain no
Comments
Phenotype verified: supE44, kanamycin resistance, and lac-.
Host for generating plasmid derivatives from recombinants in bacteriophage lambda vectors that contain lox sites flanking the plasmid portion. Example: lambdaYES.
JM107 lysogenic for lambdaKC, a bacteriophage lambda derivative containing the cre gene for the site-specific recombinase from bacteriophage P1 that recognizes lox sites. The phage also contains neo from Tn5, conferring kanamycin resistance.
Medium Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 37.0°C
Genotype endA1 gyr96 hsdR17 supE44 relA1 delta(lac-proAB) (F' traD36 proA+ proB+ lacIq delta(lacZ)M15) lambdaKC(kan-cre)
Name of Depositor SJ Elledge
References

Elledge SJ, et al. lambdaYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991. PubMed: 1848010

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation