lambdagt10 (ATCC® 40179)

Applications: Vector for constructing cDNA libraries  /  Depositors: Monsanto Co., RW Davis, PD Kelly, Monsanto Co.

Permits and Restrictions

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Designations lambdagt10
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Depositors Monsanto Co., RW Davis, PD Kelly, Monsanto Co.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host Escherichia coli BNN93 (ATCC® 47024™) (vector propagation); Escherichia coli BNN102 (ATCC® 47025™) (screen for recombinants)
Vector Information
Size (kb): 43.34
Vector: lambdagt10 (phage, lambda - replacement)
Construct size (kb): 43.34
Cloning sites: EcoRI
Cloning capacity: 7.6 kb
Host range: Escherichia coli

replicon: lambda
Vector for constructing cDNA libraries
The EcoRI site divides the vector into a left arm of 32.7 kb and a right arm of 10.7 kb.
Useful for cloning EcoRI compatible DNA fragments that range between 0-7 kb.

Huynh TV, et alConstructing and screening cDNA libraries in lambdagt10 and lambdagt11In: Huynh TV, et alDNA cloning techniques: a practical approach1OxfordIRL Presspp. 49-78, 1985

Sambrook J, et al. Molecular cloning: a laboratory manual. 1Cold Spring Harbor, NY: CSHL Press; 1989.

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