YRp7-17camp (ATCC® 37630)

Applications: YR-type (replicating) shuttle vectorshuttle vector  /  Depositors: H Feldmann

Permits and Restrictions

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Designations YRp7-17camp
Depositors H Feldmann
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.8200001716613770
Vector: YRp7-17camp (plasmid)
Promoters: Promoter cml
Construction: YRp7, pBR325
Construct size (kb): 6.820000171661377
Features: marker(s): ampR, TRP1
promoter: cml
replicon: pMB1, ARS1
terminator: CAT
YR-type (replicating) shuttle vector
shuttle vector
Restriction digests of the clone give the following sizes (kb): SalI--7.0; EcoRI--4.6, 1.4, 0.82; PvuII--4.9, 2.05.
The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al.
One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast.
A 5.47 kb ClaI/BamHI fragment of YRp7 was ligated to a 1.35 kb Sau96I fragment of pBR325. Contains the complete bacterial cml gene.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Mannhaupt G, et al. A series of shuttle vectors using chloramphenicol acetyltransferase as a reporter enzyme in yeast. Gene 67: 287-294, 1988. PubMed: 3049257

Shipped freeze-dried
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