YRp7-32cat (ATCC® 37624)

Applications: YR-type (replicating) shuttle vectorpromoter-cloning vectorshuttle vector  /  Depositors: H Feldmann

Permits and Restrictions

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Designations YRp7-32cat
Depositors H Feldmann
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.2399997711181640
Vector: YRp7-32cat (plasmid)
Promoters: Promoter none
Construction: YRp7, pBR325
Construct size (kb): 6.239999771118164
Features: insert detection: CAT
marker(s): ampR, TRP1
promoter: none
replicon: pMB1, ARS1
YR-type (replicating) shuttle vector
promoter-cloning vector
shuttle vector
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3, 1.37, 0.54; PstI--4.8, 1.4; PvuII--4.4, 1.8.
The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al.
One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast.
A 5470 bp ClaI/BamHI fragment of YRp7 was ligated to a 770 bp TaqI fragment of pBR325 containing CAT.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Mannhaupt G, et al. A series of shuttle vectors using chloramphenicol acetyltransferase as a reporter enzyme in yeast. Gene 67: 287-294, 1988. PubMed: 3049257

Shipped freeze-dried
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