YRp7-32cat (ATCC® 37624)

Applications: YR-type (replicating) shuttle vectorpromoter-cloning vectorshuttle vector  /  Depositors: H Feldmann

Permits and Restrictions

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Designations YRp7-32cat
Depositors H Feldmann
Biosafety Level 1
Vector Information
Size (kb): 6.2399997711181640
Vector: YRp7-32cat (plasmid)
Promoters: Promoter none
Construction: YRp7, pBR325
Marker(s):TRP1,ampR
Construct size (kb): 6.239999771118164
Features: insert detection: CAT
marker(s): ampR, TRP1
promoter: none
replicon: pMB1, ARS1
Applications
YR-type (replicating) shuttle vector
promoter-cloning vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3, 1.37, 0.54; PstI--4.8, 1.4; PvuII--4.4, 1.8.
The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al.
One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast.
A 5470 bp ClaI/BamHI fragment of YRp7 was ligated to a 770 bp TaqI fragment of pBR325 containing CAT.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mannhaupt G, et al. A series of shuttle vectors using chloramphenicol acetyltransferase as a reporter enzyme in yeast. Gene 67: 287-294, 1988. PubMed: 3049257

Shipped freeze-dried
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