Filobasidiella neoformans Kwon-Chung, teleomorph
34873 ™
An ampoule containing viable cells (e.g. yeast cells, spores) suspended in cryoprotectant.
- Product category
-
Fungi
- Product type
-
Yeast
- Strain designation
- NIH B-3501 [CBS 6900, CBS 7697, CBS 7817, CCRC 20532]
- Type strain
- No
- Genome sequenced strain
- Yes
- Isolation source
- Single-basidiospore isolate from a cross of ATCC 28957 x ATCC 28958.
- Product format
- Freeze-dried
- Storage conditions
-
2°C to 8°C
Documentation
ATCC determines the biosafety level of a material based on our risk assessment as guided by the current edition of Biosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. It is your responsibility to understand the hazards associated with the material per your organization’s policies and procedures as well as any other applicable regulations as enforced by your local or national agencies.
ATCC highly recommends that appropriate personal protective equipment is always used when handling vials. For cultures that require storage in liquid nitrogen, it is important to note that some vials may leak when submersed in liquid nitrogen and will slowly fill with liquid nitrogen. Upon thawing, the conversion of the liquid nitrogen back to its gas phase may result in the vial exploding or blowing off its cap with dangerous force creating flying debris. Unless necessary, ATCC recommends that these cultures be stored in the vapor phase of liquid nitrogen rather than submersed in liquid nitrogen.
Detailed product information
General
- Specific applications
-
Biomedical Research and Development Materialassay of eumelanin pigments RefWilliamson PR, et al. Melanin biosynthesis in Cryptococcus neoformans. J. Bacteriol. 180: 1570-1572, 1998. PubMed: 9515929 RefLiu L, et al. Catecholamine oxidative products, but not melanin, are produced by Cryptococcus neoformans during neuropathogenesis in mice. Infect. Immun. 67: 108-112, 1999. PubMed: 9864203produces monophenol monooxygenase phenol oxidase, phenoloxidase RefIkeda R, et al. Characterization of a phenol oxidase from Cryptococcus neoformans var. neoformans. Microbiol. Immunol. 37: 759-764, 1993. PubMed: 8289682regulation of phenol oxidase and capsular polysaccharide RefJacobson ES, Compton GM. Discordant regulation of phenoloxidase and capsular polysaccharide in Cryptococcus neoformans. J. Med. Vet. Mycol. 34: 289-291, 1996. PubMed: 8873890
- Preceptrol
- No
Characteristics
- Serotype
- D
- Mating type
-
alpha
- Genotype
- VNIV
- Morphology
- After 3 days on YM medium at 25°C, colony is cream-colored, mucoid. Cells are globose, single. No hyphae observed.
- Comments
-
Designated as type strain of Cryptococcus deneoformans Hagen et Boekhout by Hagen et al. (2015).Genome sequencing strain (The University of Oklahoma, USA).Smooth colony type RefKwon-Chung KJ. Nuclear genotypes of spore chains in Filobasidiella neoformans (Cryptococcus neoformans). Mycologia 72: 418-422, 1980. PubMed: 6991936
Handling information
- Medium
- Temperature
-
25-30°C
- Atmosphere
-
Aerobic
- Handling procedure
-
For freeze-dry (lyophilized) ampoules:
- Open an ampoule according to enclosed instructions.
- From a single test tube of sterile distilled water (5 to 6 mL), withdraw approximately 0.5 to 1.0 mL with a sterile pipette and apply directly to the pellet. Stir to form a suspension.
- Aseptically transfer the suspension back into the test tube of sterile distilled water.
- Let the test tube sit at room temperature (25°C) undisturbed for at least 2 hours; longer (e.g., overnight) rehydration might increase viability of some fungi.
- Mix the suspension well. Use several drops (or make dilutions if desired) to inoculate recommended solid or liquid medium. Include a control that receives no inoculum.
- Incubate the inoculum at the propagation conditions recommended.
- Inspect for growth of the inoculum/strain regularly. The sign of viability is noticeable typically after 1-2 days of incubation. However, the time necessary for significant growth will vary from strain to strain.
- Handling notes
- Additional information on this culture is available on the ATCC® web site at www.atcc.org.
Quality control specifications
- Sequenced data
-
18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence
GGTTTCCGTAGGTGAACCTGCGGAAGGATCAGTAGAGAATATTGGACTTTGGTCCATTTATCTACCCATCTACACCTGTGAACTGTTTATGTGCTTCGGCACGTTTTACACAAACTTCTAAATGTAATGAATGTAATCATATTATAACAATAATAAAACTTTCAACAACGGATCTCTTGGCTTCCACATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCAACTTGCGCCCTTTGGTATTCCGAAGGGCATGCCTGTTTGAGAGTCATGAAAATCTCAATCCCTCGGGTTTTATTACCTGTTGGACTTGGATTTGGGTGTTTGCCGCGACCTGCAAAGGACGTCGGCTCGCCTTAAATGTGTTAGTGGGAAGGTGATTACCTGTCAGCCCGGCGTAATAAGTTTCGCTGGGCCTATGGGGTAGTCTTCGGCTTGCTGATAACAACCATCTCTTTTTGTTTGACCTCAAATCAGGTAGGGCTACCCGCTGAACTTAAGCATATCAATAA
D1D2 region of the 26S ribosomal RNA geneATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGTGAACCGGGAAGAGCTCAAATTTGAAATCTGGCGTCCTCCGGGCGTCCGAGTTGTAATCTACAGAAACGTTTTCCGTGCTGGACCGTGTCTAAGTCCCTTGGAATAGGGTATCAAAGAGGGTGACAATCCCGTACTTGACACGATCACCAGTGCTCTGTGATACGTTTTCTACGAGTCGCGTTACTTGGGAGTGTAGCGCAAAATGGGTGGTAAACTCCATCTAAAGCTAAATATTGGTGGAAGACCGATAGCGAACAAGTACCGTGAGGGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAACGATTGAAGTCAGTCGTGTCTATTGGGTTCAGCCAGTTCTGCTGGTGTATTCCCTTTAGACGGGTCAACATCAGTTCTGATCGGTGGATAAGGGCTGGAGGAATGTGGCACTCTTCGGGGTGTGTTATAGCCTCCTGTCGCATACACTGGTTGGGACTGAGGAATGCAGCTCGCCTTTATGGCCGGGGTTCGCCCACGTTCGAGCTTAGGATGTTGACAAAATGGCTTTAAACGAC
26S ribosomal RNA gene and intergenic spacer (IGS), partial sequenceATGGGAACGGGGTGCTGTAAGTGGTAGAGTAGCCTTGTTGCTACGATCCACTGAGGCTAAGCCCTTGTTCTATAGATTTGTCTCTAACATGTTGGGTCTCGGGGGGCTTCCTCTAGAGACTTGGATGTAAGGGGCTTTACGCATTCACATAATATTAGTTGGGGACTGAGGGGGGTGTGTGCTGTGCGAGCCCGGCTGATAATTGTTTTAATTGTTAGCTTGGATTGCACACACAGTCTCATCAGTCTTCAGCTTGGGTTATCGATATTCTGTAAGTCCAAACTTATCCATTCACATTCACTGTAGTGTAGGTTGCAGAGAGGACTTGGAAAGTTCTCTCTCTGCACTGGGCATACTACTGATTTTATGGTCATGGGGGACTTGGGAGCTGGTGCTTGTGTCGCATAAGGTAGAGTTGATGGTTGGACAGATAGATAAGCATTTTTATGTCGCGGGGGACTTGGGGTAAGACGCCTTGCAGCAAGTAAGAGAGTCAAACAGTAGTTACTTGTTCTAGTCGTGGGGGACTTTGGTAGTGGTAGTGAGCCGGAATGAGAGATGGATAAATGCAGTATGGATGGTGACAGGAGGAGCAGCAGAGCGTATGCAGTGGATATGTATATAGTTGAGAAGGGATGGACAGTAGAACGTTTGAACGTTGAGGAATAATTGACCCAAGGGTGATTCTAATAGACTTGTCAAGAAAAAGTTAAGTTGAATCAAGCTTGGTCAAGCAAAGTCTAGAAAAGATTTACAATCACCAAGAATTGCCCGAGTCGCGGGGGGCTTGTCACTTCTCTTGCTCATTGTGGGTCCAGTCT
glycerol-3-phosphate dehydrogenase 1 (GPD1) gene, partial cdsCAACGGTTTCGGTACGTAATCAATCCTTCTCTTCCCGTTGCTTCATATCCCACCGATGTGACTTCCAATATTGTGCGCTGGCTGTTCTTGAGTTATGGTAGCTAATAGCTATGTGATAGGTCGTATTGGTCGAATTGTTCTCAGGTATGCTTTCTTTAAGTCAACGGGGTAAGAAGCCCGTGTATCTAACGTTCAGGTAGGAACGCCATCGAGCATGGGGACCTCGAGGTTGTTGCTGTCAACGAGTGAGTGTCCCAAAAGTGTTAATCCATTAGATGAATCACTTAATTGTCCATAGCCCTTTTATTGACTTAGACTACATGGTATGATACTTCTTTCCGCCCAATAGCTTCGTACTGACTCTGTTGCGCAGGTCTACATGTTCAAGTATGACTCCGTAAGTCTCCCAAGTTCTAACGTTCTGTATGGCTTATTAACCTGATTTTCGACAGACACATGGTCGCTTCAAGGGCTCCGTCGAGGTTAAGGACGGTAAACTTTACATCAACAACAAGGCCATTGCCGTCTTCGGCGAGAAAGACCCTGCCAACATCAAGTGGGG
History
- Deposited as
- Cryptococcus neoformans (Sanfelice) Vuillemin
- Synonyms
- Filobasidiella bacillispora Kwon-Chung, teleomorph; Torula nasalis Harrison, teleomorph
- Depositors
- KJ Kwon-Chung
- Cross references
-
GenBank AF316889 Filobasidiella neoformans var. neoformans elongation factor 3 mRNA, complete cds.
Legal disclaimers
- Intended use
- This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use.
- Warranty
-
The product is provided 'AS IS' and the viability of ATCC® products is warranted for 30 days from the date of shipment, provided that the customer has stored and handled the product according to the information included on the product information sheet, website, and Certificate of Analysis. For living cultures, ATCC lists the media formulation and reagents that have been found to be effective for the product. While other unspecified media and reagents may also produce satisfactory results, a change in the ATCC and/or depositor-recommended protocols may affect the recovery, growth, and/or function of the product. If an alternative medium formulation or reagent is used, the ATCC warranty for viability is no longer valid. Except as expressly set forth herein, no other warranties of any kind are provided, express or implied, including, but not limited to, any implied warranties of merchantability, fitness for a particular purpose, manufacture according to cGMP standards, typicality, safety, accuracy, and/or noninfringement.
- Disclaimers
-
This product is intended for laboratory research use only. It is not intended for any animal or human therapeutic use, any human or animal consumption, or any diagnostic use. Any proposed commercial use is prohibited without a license from ATCC.
While ATCC uses reasonable efforts to include accurate and up-to-date information on this product sheet, ATCC makes no warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. ATCC does not warrant that such information has been confirmed to be accurate or complete and the customer bears the sole responsibility of confirming the accuracy and completeness of any such information.
This product is sent on the condition that the customer is responsible for and assumes all risk and responsibility in connection with the receipt, handling, storage, disposal, and use of the ATCC product including without limitation taking all appropriate safety and handling precautions to minimize health or environmental risk. As a condition of receiving the material, the customer agrees that any activity undertaken with the ATCC product and any progeny or modifications will be conducted in compliance with all applicable laws, regulations, and guidelines. This product is provided 'AS IS' with no representations or warranties whatsoever except as expressly set forth herein and in no event shall ATCC, its parents, subsidiaries, directors, officers, agents, employees, assigns, successors, and affiliates be liable for indirect, special, incidental, or consequential damages of any kind in connection with or arising out of the customer's use of the product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, ATCC is not liable for damages arising from the misidentification or misrepresentation of such materials.
Please see the material transfer agreement (MTA) for further details regarding the use of this product. The MTA is available at www.atcc.org.
Permits & Restrictions
Import Permit for the State of Hawaii
If shipping to the U.S. state of Hawaii, you must provide either an import permit or documentation stating that an import permit is not required. We cannot ship this item until we receive this documentation. Contact the Hawaii Department of Agriculture (HDOA), Plant Industry Division, Plant Quarantine Branch to determine if an import permit is required.
Frequently Asked Questions
References
Curated Citations
Salas SD, et al. Effect of the laccase gene, CNLAC1, on virulence of cryptococcus neoformans. J. Exp. Med. 184: 377-386, 1996. PubMed: 8760791
Schmeding KA, et al. Sexual compatibility between serotypes of Filobasidiella neoformans (Cryptococcus neoformans). Curr. Microbiol. 5: 133-138, 1981.
Kwon-Chung KJ. Morphogenesis of Filobasidiella neoformans, the sexual state of Cryptococcus neoformans. Mycologia 68: 821-833, 1976. PubMed: 790172
Kwon-Chung KJ. Nuclear genotypes of spore chains in Filobasidiella neoformans (Cryptococcus neoformans). Mycologia 72: 418-422, 1980. PubMed: 6991936
Jacobson ES, Compton GM. Discordant regulation of phenoloxidase and capsular polysaccharide in Cryptococcus neoformans. J. Med. Vet. Mycol. 34: 289-291, 1996. PubMed: 8873890
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