Schizosaccharomyces pombe Lindner (ATCC® 201437)

Strain Designations: FY191  /  Product Format: frozen

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Deposited As Schizosaccharomyces pombe Lindner, teleomorph
Strain Designations FY191
Application
produces protein tetracycline repressor
transformation host
host required for regulated expression of sequences cloned into vectors containing a tet operator and a CaMV promoter (pSLF101, ATCC 87619; pSLF102, ATCC 87620).
The CaMV-tet vector in the strain FY191 is repressed in the absence of tetracycline and induced by addition of tetracycline to 10 ug/ml.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Genotype h- ura4-D18 leu1-32 ade6-704::pSLF104 [sup3-5 adh-tetR]
Preceptrol® no
Mating Type h-
Ploidy haploid
Comments
The vector contains the marker sup3-5, which suppresses ade6-704, and the tet repressor gene under control of the S. pombe adh promoter.
produces the tetracycline repressor constitutively
Medium ATCC® Medium 2064: YES MEDIUM
Growth Conditions
Temperature: 25.0°C
Name of Depositor SL Forsburg
Special Collection NCRR Contract
Isolation
generated by integrating the vector pSLF104 (ATCC 87621) into a strain of genotype h- ade6-704 leu1-32 ura4-D18
References

Forsburg SL. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21: 2955-2956, 1993. PubMed: 8332516

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation