Schizosaccharomyces pombe Lindner, teleomorph (ATCC® 201437)

Strain Designations: FY191  /  Product Format: frozen

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Strain Designations FY191
Application
produces protein tetracycline repressor
transformation host
host required for regulated expression of sequences cloned into vectors containing a tet operator and a CaMV promoter (pSLF101, ATCC 87619; pSLF102, ATCC 87620).
The CaMV-tet vector in the strain FY191 is repressed in the absence of tetracycline and induced by addition of tetracycline to 10 ug/ml.
Biosafety Level 1
Product Format frozen
Storage Conditions Frozen: -80°C or colder
Freeze-Dried: 2°C to 8°C
Live Culture: See Propagation Section
Type Strain no
Genotype h- ura4-D18 leu1-32 ade6-704::pSLF104 [sup3-5 adh-tetR]
Preceptrol® no
Mating Type h-
Comments
The vector contains the marker sup3-5, which suppresses ade6-704, and the tet repressor gene under control of the S. pombe adh promoter.
produces the tetracycline repressor constitutively
Medium ATCC® Medium 2064: YES MEDIUM
Growth Conditions
Temperature: 25.0°C
Name of Depositor SL Forsburg
Special Collection NCRR Contract
Isolation
generated by integrating the vector pSLF104 (ATCC 87621) into a strain of genotype h- ade6-704 leu1-32 ura4-D18
References

Forsburg SL. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res. 21: 2955-2956, 1993. PubMed: 8332516

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation