plambda500 [p500] (ATCC® 57212)

Organism: Homo sapiens, human  /  Clone Type: Clone  /  Depositors: C Caskey, PI Patel

Permits and Restrictions

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Designations plambda500 [p500]
Species Homo sapiens, human
Depositors C Caskey, PI Patel
Vector
Construct size (kb): 10.25
Insert
DNA: genomic
Insert lengths(kb): 5.900000095367432
Gene product: hypoxanthine phosphoribosyltransferase pseudogene 2 [HPRTP2]
Alleles: A1, A2
Insert Size (kb) 5.900
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Shipping Information Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.1 kb doublet; HindIII--10.25; PstI--10.25; EcoRI/BamHI--5.6, 4.1, 1.25; EcoRI/HindIII--5.1, 4.4, 0.9.
The 0.9 kb EcoRI/HindIII fragment represents the HPRTP2 gene.
The insert includes a 5.0 kb EcoRI fragment and a 0.9 kb EcoRI/HindIII fragment, with the latter subcloned from Hulambda5.
The 3.8 kb constant band is from the X chromosome.
Enzyme(s) not detecting polymorphism: TaqI, PstI, BstNI, XbaI, SacI, PvuII, HindIII, HincII, StuII, MboI, EcoRV.
References

Weiffenbach B, et al. A genetic linkage map of human chromosome 5 with 60 RFLP loci. Genomics 10: 173-185, 1991. PubMed: 1675192

Patel PI, et al. Organization of the HPRT gene and related sequences in the human genome. Somatic Cell Mol. Genet. 10: 483-493, 1984. PubMed: 6089358

C. Thomas Caskey, personal communication

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