Chlamydia trachomatis (ATCC® VR-1477)

Classification: Chlamydiaceae, Chlamydia  /  Product Format: frozen 1 mL per vial

Classification Chlamydiaceae, Chlamydia
Agent Chlamydia trachomatis
Strain TW-3 [Trachoma type C strain TW-3]
Applications
Sexually Transmitted Disease Research
Positive for Mycoplasma Yes
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments

FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.

This strain is a tissue culture-adapted version of strain TW-3 (VR-578) which is grown in embryonated chicken eggs.

Before infecting host cells, add 1-mm glass beads and vortex stock material for 45 seconds. Aspirate fluid. Infect shell vial monolayer with 0.2 mL of material.
Centrifuge at 800 x G for 1 hour. Feed with 1.0 mL EMEM (HEPES buffered), 10% FBS (screened for chlamydia antibodies).
Serotype C
Effect on Host
Requires FA to visualize intracellular inclusion bodies
Recommended Host
HEp-2 cells (ATCC CCL-23)
Growth Conditions
Temperature: 37°C
Recommendations for Infection: Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour. Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2mg/mL cycloheximide. Incubate at 37°C for 48 to 72 hours, a 5% CO2 in air atmosphere is recommended.
Incubation: 48 to 72 hours, a 5% CO2 in air atmosphere is recommended.
Effect on Host
Requires FA to visualize intracellular inclusion bodies
Name of Depositor Kuo
Special Collection ATCC
Source Specimen from human conjunctiva, Taiwan
References

Grayston JT, et al. Trachoma-studies of etiology, laboratory diagnosis, and prevention. JAMA 172: 1577-1586, 1960. PubMed: 13829148