pfdB2 (ATCC® 37371)

Applications: vector permitting production of single-stranded DNA  /  Depositors: K Geider

Permits and Restrictions

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Designations pfdB2
Depositors K Geider
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 1.8899999856948850
Vector: pfdB2 (plasmid)
Promoters: Promoter none
Construction: pfdA2, pIG-414RB
Marker(s):kanR
Construct size (kb): 1.889999985694885
Features: marker(s): kanR
promoter: none
replicon: fd
terminator: none
enhancer: none
Applications
vector permitting production of single-stranded DNA
Comments
The plasmid is lost at 42C if grown without antibiotic. The host Escherichia coli strain must express gene 2 of filamentous bacteriophages.
Vector with bacteriophage fd replication system.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Geider K, et al. A plasmid cloning system utilizing replication and packaging functions of the filamentous bacteriophage fd. Gene 33: 341-349, 1985. PubMed: 2989095

Shipped freeze-dried