pfdB2 (ATCC® 37371)

Applications: vector permitting production of single-stranded DNA  /  Depositors: K Geider

Permits and Restrictions

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Designations pfdB2
Depositors K Geider
Biosafety Level 1
Vector Information
Size (kb): 1.8899999856948850
Vector: pfdB2 (plasmid)
Promoters: Promoter none
Construction: pfdA2, pIG-414RB
Marker(s):kanR
Construct size (kb): 1.889999985694885
Features: marker(s): kanR
promoter: none
replicon: fd
terminator: none
enhancer: none
Applications
vector permitting production of single-stranded DNA
Comments
The plasmid is lost at 42C if grown without antibiotic. The host Escherichia coli strain must express gene 2 of filamentous bacteriophages.
Vector with bacteriophage fd replication system.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C
References

Geider K, et al. A plasmid cloning system utilizing replication and packaging functions of the filamentous bacteriophage fd. Gene 33: 341-349, 1985. PubMed: 2989095

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet