Size (kb): 7.0000000000000000
Vector: pNF3 (plasmid)
Promoters: Promoter nifH
Construct size (kb): 7.0
Features: marker(s): ampR, kanR, G418R, URA3
marker(s): ampR, kanR, spcR, strR
YI-type (integrating) shuttle vector
This plasmid is identical to pNF2 (ATCC 37187)
except for the deletion of the 2.2 kb EcoRI fragment containing the yeast 2 micron origin of replication.
SalI sites flank the BamHI cloning site for ready removal of the insert.
A integration shuttle vector for subcloning and recovery of DNA from Saccharomyces cerevisiae.
Naumovski L, Friedberg EC. Construction of plasmid vectors that facilitate subcloning and recovery of yeast and Escherichia coli DNA fragments. Gene 22: 203-209, 1983. PubMed: 6307821
Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863