pNF3 (ATCC® 37188)

Applications: YI-type (integrating) shuttle vectorintegrating vectorshuttle vector  /  Depositors: EC Friedberg

Permits and Restrictions

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Designations pNF3
Depositors EC Friedberg
Biosafety Level 1
Vector Information
Size (kb): 7.0000000000000000
Vector: pNF3 (plasmid)
Promoters: Promoter nifH
Construction: pNF2
Marker(s):G418R,URA3,ampR,kanR
Construct size (kb): 7.0
Features: marker(s): ampR, kanR, G418R, URA3
marker(s): ampR, kanR, spcR, strR
promoter: nifH
replicon: pMB1
terminator: none
Applications
YI-type (integrating) shuttle vector
integrating vector
shuttle vector
Comments
This plasmid is identical to pNF2 (ATCC 37187) except for the deletion of the 2.2 kb EcoRI fragment containing the yeast 2 micron origin of replication.
SalI sites flank the BamHI cloning site for ready removal of the insert.
A integration shuttle vector for subcloning and recovery of DNA from Saccharomyces cerevisiae.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Naumovski L, Friedberg EC. Construction of plasmid vectors that facilitate subcloning and recovery of yeast and Escherichia coli DNA fragments. Gene 22: 203-209, 1983. PubMed: 6307821

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Shipped frozen
Product Sheet
Product Sheet
Product Sheet