pNF3 (ATCC® 37188)

Applications: YI-type (integrating) shuttle vectorintegrating vectorshuttle vector  /  Depositors: EC Friedberg

Permits and Restrictions

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Designations pNF3
Depositors EC Friedberg
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 7.0000000000000000
Vector: pNF3 (plasmid)
Promoters: Promoter nifH
Construction: pNF2
Marker(s):G418R,URA3,ampR,kanR
Construct size (kb): 7.0
Features: marker(s): ampR, kanR, G418R, URA3
marker(s): ampR, kanR, spcR, strR
promoter: nifH
replicon: pMB1
terminator: none
Applications
YI-type (integrating) shuttle vector
integrating vector
shuttle vector
Comments
This plasmid is identical to pNF2 (ATCC 37187) except for the deletion of the 2.2 kb EcoRI fragment containing the yeast 2 micron origin of replication.
SalI sites flank the BamHI cloning site for ready removal of the insert.
A integration shuttle vector for subcloning and recovery of DNA from Saccharomyces cerevisiae.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Naumovski L, Friedberg EC. Construction of plasmid vectors that facilitate subcloning and recovery of yeast and Escherichia coli DNA fragments. Gene 22: 203-209, 1983. PubMed: 6307821

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Shipped frozen
Product Sheet
Product Sheet
Product Sheet