pRSVcat (ATCC® 37152)

Applications: expression vectorshuttle vector  /  Depositors: BH Howard

Permits and Restrictions

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Designations pRSVcat
Depositors BH Howard
Biosafety Level 1
Vector Information
Size (kb): 5.1
Vector: pRSVcat (plasmid)
Promoters: Promoter RSV LTR
Construction: pSV2-betaG, Tn9, RSV
Marker(s):ampR,cmlR
Construct size (kb): 5.1
Features: marker(s): ampR, cmlR
promoter: RSV LTR
replicon: pMB1
enhancer: RSV
Applications
expression vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): PvuII--5.1; BamHI--5.1; PstI--4.1, 1.0.
Restriction digests of the clone give the following sizes (kb): HindIII--5.0; BglII--uncut; BamHI--5.0.
Vector for expression of chloramphenicol acetyltransferase in eukaryotic cell lines under the control of Rous Sarcoma Virus long terminal repeats.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Gorman CM, et al. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol. Cell. Biol. 2: 1044-1051, 1982. PubMed: 6960240

Gorman CM, et al. The rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection. Proc. Natl. Acad. Sci. USA 79: 6777-6781, 1982. PubMed: 6294651

Gorman C, et al. High efficiency DNA-mediated transformation of primate cells. Science 221: 551-553, 1983. PubMed: 6306768

Shipped freeze-dried
Shipping Information

Shipped:  Freeze-dried Escherichia coli HB101 containing the plasmid.

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