pRSVcat (ATCC® 37152)

Applications: expression vectorshuttle vector  /  Depositors: BH Howard

Permits and Restrictions

View Permits

Designations pRSVcat
Depositors BH Howard
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.1
Vector: pRSVcat (plasmid)
Promoters: Promoter RSV LTR
Construction: pSV2-betaG, Tn9, RSV
Construct size (kb): 5.1
Features: marker(s): ampR, cmlR
promoter: RSV LTR
replicon: pMB1
enhancer: RSV
expression vector
shuttle vector
Restriction digests of the clone give the following sizes (kb): PvuII--5.1; BamHI--5.1; PstI--4.1, 1.0.
Restriction digests of the clone give the following sizes (kb): HindIII--5.0; BglII--uncut; BamHI--5.0.
Vector for expression of chloramphenicol acetyltransferase in eukaryotic cell lines under the control of Rous Sarcoma Virus long terminal repeats.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Gorman CM, et al. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol. Cell. Biol. 2: 1044-1051, 1982. PubMed: 6960240

Gorman CM, et al. The rous sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA-mediated transfection. Proc. Natl. Acad. Sci. USA 79: 6777-6781, 1982. PubMed: 6294651

Gorman C, et al. High efficiency DNA-mediated transformation of primate cells. Science 221: 551-553, 1983. PubMed: 6306768

Shipped freeze-dried
Shipping Information

Shipped:  Freeze-dried Escherichia coli HB101 containing the plasmid.