YIp1 [pRB14] (ATCC® 37063)

Applications: YI-type (integrating) shuttle vectorintegrating vectorshuttle vector  /  Depositors: D Botstein

Permits and Restrictions

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Designations YIp1 [pRB14]
Depositors D Botstein
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 9.8000001907348630
Vector: YIp1 (plasmid)
Construction: pBR322, pGT2-Sc2605
Construct size (kb): 9.800000190734863
Features: marker(s): ampR, HIS3
replicon: pMB1
YI-type (integrating) shuttle vector
integrating vector
shuttle vector
Restriction digests analyzed on agarose gels give the following sizes (kb): BamHI--9.0, 1.5; EcoRI--9.8.
This construct is not stable when freeze-dried and is distributed from liquid nitrogen stock.
A general purpose integrating Saccharomyces cerevisiae/Escherichia coli shuttle vector.
This was constructed by inserting a 6.1 kb EcoRI/SalI HIS3-containing fragment from pGT2-Sc2605 into pBR322. The HIS3 fragment was originally isolated from the diploid strain A364a X H79-20.3alpha.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Shipped frozen