YIp1 [pRB14] (ATCC® 37063)

Applications: YI-type (integrating) shuttle vectorintegrating vectorshuttle vector  /  Depositors: D Botstein

Permits and Restrictions

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Designations YIp1 [pRB14]
Depositors D Botstein
Biosafety Level 1
Vector Information
Size (kb): 9.8000001907348630
Vector: YIp1 (plasmid)
Construction: pBR322, pGT2-Sc2605
Marker(s):ampR,HIS3
Construct size (kb): 9.800000190734863
Features: marker(s): ampR, HIS3
replicon: pMB1
Applications
YI-type (integrating) shuttle vector
integrating vector
shuttle vector
Comments
Restriction digests analyzed on agarose gels give the following sizes (kb): BamHI--9.0, 1.5; EcoRI--9.8.
This construct is not stable when freeze-dried and is distributed from liquid nitrogen stock.
A general purpose integrating Saccharomyces cerevisiae/Escherichia coli shuttle vector.
This was constructed by inserting a 6.1 kb EcoRI/SalI HIS3-containing fragment from pGT2-Sc2605 into pBR322. The HIS3 fragment was originally isolated from the diploid strain A364a X H79-20.3alpha.
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Shipped frozen
Product Sheet
Product Sheet
Product Sheet