mAF-1/7 (ATCC® 61121)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations mAF-1/7
Species Homo sapiens, human
Vector
Construct size (kb): 7.199999809265137
Insert
DNA: genomic
Insert lengths(kb): 0.5
Tissue: placenta
Gene product: immunoglobulin kappa variable region [IGKV]
Alleles: Gd, Nd, Gd, Gd, Gd, Gp, Nd, Nd, Np, Np, Np, Gp, Gp, Nd, Gd, Nd, Gd, Nd, Gd, Nd, Gd, Nd, Np, Gp, Np, Gp, Np, Gp, Np, Gp
Insert Size (kb) 0.5
Biosafety Level 1
Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 2 ug)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/SpnI--7.2, 0.5; EcoRI/HindIII--7.2, 0.54; EcoRI--7.8; BamHI--7.8; HindIII--7.8.
This insert includes part of the intron between the leader and the V gene segment as well as sequences encoding the FR1, CDR1, FR2, CDR2, FR3, and part of CDR3. This is in the germline configuration and encodes a Vkappa of subgroup IV.
References

Klobeck HG, et al. Subgroup IV of human immunoglobulin kappa light chains is encoded by a single germline gene. Nucleic Acids Res. 13: 6515-6529, 1985. PubMed: 2997712

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