p11-1-111 (ATCC® 59017)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations p11-1-111
Species Homo sapiens, human
Vector
Construct size (kb): 8.600000381469727
Insert
DNA: genomic
Insert lengths(kb): 3.0
Gene product: DNA Segment, single copy [D12S6]
Alleles: A1, A1, A2, A2, C1, C2, D1, D2
Insert Size (kb) 3.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA distributed as 5 ug in 50 ul TE (volume: 50 ul,
Comments
Restriction digests of the clone give the following sizes (kb): AvaI--6.5, 2.8; SalI--9.3; PstI--6.4, 1.2 (doublet); HindIII--5.8, 3.1; EcoRI--5.6, 3.0.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
This is one of 4 probes from cosmid 1-11 which reveals RFLPs at D12S6. Using all 4 probes, the PIC of the locus is 0.73. This polymorphism is also detected by BamHI and HindIII.
Constructed by inserting Sau3A fragment of cosmid 1-11 into BamHI site.
References

Buroker NE, et al. Four restriction length polymorphisms revealed by probes from a single cosmid map to human chromosome 12q. Hum. Genet. 72: 86-94, 1986. PubMed: 3002956

O'Connell P, et al. A primary genetic linkage map for human chromosome 12. Genomics 1: 93-102, 1987. PubMed: 3478299

Michael Litt, personal communication

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