p11-1-111 (ATCC® 59017)

Organism: Homo sapiens, human  /  Clone Type: Clone  / 

Permits and Restrictions

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Designations p11-1-111
Species Homo sapiens, human
Vector
Construct size (kb): 8.600000381469727
Insert
DNA: genomic
Insert lengths(kb): 3.0
Gene product: DNA Segment, single copy [D12S6]
Alleles: A1, A1, A2, A2, C1, C2, D1, D2
Insert Size (kb) 3.0
Biosafety Level 1
Shipping Information Distributed: DNA distributed as 5 ug in 50 ul TE (volume: 50 ul,
Comments
Restriction digests of the clone give the following sizes (kb): AvaI--6.5, 2.8; SalI--9.3; PstI--6.4, 1.2 (doublet); HindIII--5.8, 3.1; EcoRI--5.6, 3.0.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
This is one of 4 probes from cosmid 1-11 which reveals RFLPs at D12S6. Using all 4 probes, the PIC of the locus is 0.73. This polymorphism is also detected by BamHI and HindIII.
Constructed by inserting Sau3A fragment of cosmid 1-11 into BamHI site.
References

Buroker NE, et al. Four restriction length polymorphisms revealed by probes from a single cosmid map to human chromosome 12q. Hum. Genet. 72: 86-94, 1986. PubMed: 3002956

O'Connell P, et al. A primary genetic linkage map for human chromosome 12. Genomics 1: 93-102, 1987. PubMed: 3478299

Michael Litt, personal communication

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