pbPKC-beta15-E1 [pbPKC-beta15-EcoRI-1] (ATCC® 37706)

Organism: Bos taurus, cow  /  Clone Type: Clone  /  Depositors: A Ullrich

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Designations pbPKC-beta15-E1 [pbPKC-beta15-EcoRI-1]
GenBank Number

M13974

Species Bos taurus, cow
Depositors A Ullrich
Applications
Detects a human genomic HindIII fragment of 7.5 kb and was used to map the human gene.
Vector
Construct size (kb): 4.619999885559082
Insert
DNA: cDNA
Insert lengths(kb): 1.921000003814697
Tissue: brain
Gene product: protein kinase C, beta 1(protein kinase C, beta I) [PRKCB]
Insert Size (kb) 1.921
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1
Comments
Contains the following restriction sites (with nucleotide positions according to the published sequence): PstI--231, BamHI--623, PstI--865, PstI--1408.
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7, 2.0; PstI--3.3, 0.7, 0.6; BamHI--4.4, 0.6; HindIII--5.0.
This is the 5' EcoRI subclone of pbPKC-beta15 and extends from nt 105 to nt 2027 of the published sequence, including most of the coding sequence which extends from nt 65 to nt 2084.
Detects bovine cerebellar mRNA of 8.7, 3.4, and 2.6 kb, and rat brain mRNA of 8.7 and 2.6 kb.
Detects a human genomic HindIII fragment of 7.5 kb and was used to map the human gene.
References

Coussens L, et al. Multiple, distinct forms of bovine and human protein kinase C suggest diversity in cellular signaling pathways. Science 233: 859-866, 1986. PubMed: 3755548

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