A-704 (ATCC® HTB-45)

Organism: Homo sapiens, human  /  Tissue: kidney  /  Disease: adenocarcinoma

Organism Homo sapiens, human
Tissue
kidney
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Disease adenocarcinoma
Age 78 years
Gender male
Applications
This cell line is a suitable transfection host. ref
Storage Conditions liquid nitrogen vapor phase
Karyotype (P59) diploid to hyperdiploid, hypertriploid to hypertetraploid with abnormalities including breaks, dicentrics and endoreduplication
Derivation
The A-704 line was derived by D.J. Giard as indicated in the description for ATCC HTB-41.
Clinical Data
male
Tumorigenic No
Effects
No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37°C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Cryopreservation
Culture medium, 95%; DMSO, 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 7,8
D13S317: 8
D16S539: 12,13
D5S818: 10,11
D7S820: 10
THO1: 7,9
TPOX: 11
vWA: 14,18
Isoenzymes
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1-2
Name of Depositor DJ Giard
References

Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758

Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047

Cross References

Nucleotide (GenBank) : U02327 Human clone ndf19 neu differentiation factor mRNA, partial cds.

Nucleotide (GenBank) : U02330 Human clone ndf33 neu differentiation factor mRNA, partial cds.

Nucleotide (GenBank) : U02326 Human clone ndf43 neu differentiation factor mRNA, complete cds.

Basic Documentation
References

Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758

Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047