1G2 (ATCC® CRL-13005)

Organism: Homo sapiens, human  /  Cell Type: epithelial; somatic cell hybri  /  Disease: Burkitt's lymphoma

Organism Homo sapiens, human
Cell Type epithelial; somatic cell hybri
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 cells contain Ad5, SV40, and EBV viral DNA sequences
Disease Burkitt's lymphoma
Applications
The 1G2 cell line was established by transfection of HKB-11 cells (ATCC CRL-12568) with pSH157 expression vector coding for an IL-4 selective agonist, IL-4SA. This cell line can be used for relatively quick production of small amounts of IL-4SA protein (gram quantities).
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
The 1G2 cell line was established by transfection of HKB-11 cells (ATCC CRL-12568) with pSH157 expression vector coding for an IL-4 selective agonist, IL-4SA. This cell line can be used for relatively quick production of small amounts of IL-4SA protein (gram quantities). The 1G2 cell line was deposited at the ATCC and is available as PTA-87.
Comments
The 1G2 cell line was established by transfection of HKB-11 cells (ATCC CRL-12568) with pSH157 expression vector coding for an IL-4 selective agonist, IL-4SA. This cell line can be used for relatively quick production of small amounts of IL-4SA protein (gram quantities). The 1G2 cell line was deposited at the ATCC and is available as PTA-87.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
Subculturing
Protocol: Cells will slough off easily, subculture before confuency
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.
Subcultivation Ratio: 1:6 to 1:10 once or twice weekly
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Name of Depositor Bayer Corporation
References

Cho MS. Human hybrid host cell for mammalian gene expression . US Patent 6,136,599 dated Oct 24 2000

Cho MS, et al. Expression system for factor VIII. US Patent 6,358,703 dated Mar 19 2002

Basic Documentation
Other Documentation
References

Cho MS. Human hybrid host cell for mammalian gene expression . US Patent 6,136,599 dated Oct 24 2000

Cho MS, et al. Expression system for factor VIII. US Patent 6,358,703 dated Mar 19 2002