TK6 (ATCC® CRL-8015)

Organism: Homo sapiens, human  /  Cell Type: lymphoblast  /  Tissue: spleen  /  Disease: hereditary spherocytosis

Organism Homo sapiens, human
Tissue
spleen
Cell Type lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2
Disease hereditary spherocytosis
Age 5 years
Gender male
Applications
The cells are heterozygous at the thymidine kinase (TK) locus and can be used to quantitatively detect forward mutations at 3 loci (resistance to trifluorothymidine (tk locus)).
Storage Conditions liquid nitrogen vapor phase
Derivation
This line is derivative of the WIL-2 cell line.
Clinical Data
male
Comments
Resistance to thioguanine (hprt locus), and resistance to ouabain (Na/K ATPase).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Maintain the cell concentration between 2 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days (or as necessary to maintain the cell concentration between 2 X 105 and 1 X 106 cells/ml)
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 9,11
THO1: 8,9.3
TPOX: 8,11
vWA: 17,20
Name of Depositor Massachusetts Institute of Technology
References

Skopek TR, et al. Assay for mutagenesis in heterozygous diploid human lymphoblasts. US Patent 4,302,535 dated Nov 24 1981

Skopek TR, et al. Isolation of a human lymphoblastoid line heterozygous at the thymidine kinase locus: possibility for a rapid human cell mutation assay. Biochem. Biophys. Res. Commun. 84: 411-416, 1978. PubMed: 214074

Levy JA, et al. Human lymphoblastoid lines from lymph node and spleen. Cancer 22: 517-524, 1968. PubMed: 5673231

Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642

Basic Documentation
References

Skopek TR, et al. Assay for mutagenesis in heterozygous diploid human lymphoblasts. US Patent 4,302,535 dated Nov 24 1981

Skopek TR, et al. Isolation of a human lymphoblastoid line heterozygous at the thymidine kinase locus: possibility for a rapid human cell mutation assay. Biochem. Biophys. Res. Commun. 84: 411-416, 1978. PubMed: 214074

Levy JA, et al. Human lymphoblastoid lines from lymph node and spleen. Cancer 22: 517-524, 1968. PubMed: 5673231

Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642