WBE (ATCC® CRL-2773)

Organism: Morone chrysops, white bass  /  Cell Type: epithelial  / 

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Organism Morone chrysops, white bass
Cell Type epithelial
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 1
Age 24 hours post fertilization embryo
Gender unknown
Applications
These cells are useful for the study of host-pathogen interactions.
Storage Conditions liquid nitrogen vapor phase
Karyotype Aneuploid. Range = 44 to 49. The chromosme morphology was normal for bass with all telocentric and acrocentric chromosomes. No dicentric or ring chromosomes were observed. RefShimizu C, et al. Characterization of a white bass (Morone chrysops) embryonic cell line with epithelial features. In Vitro Cell. Dev. Biol. Anim. 39: 29-35, 2003. PubMed: 12892524
Derivation
San Diego California,
Clinical Data
unknown
Genes Expressed
keratin
Effects
No, does not form colonies in soft agar
Comments
These cells are useful for the study of host-pathogen interactions.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 31°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 31°C.
Subcultivation Ratio: 1:4 to 1:6 weekly
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 31.0°C
Name of Depositor M Westerman
Year of Origin February 22, 2001
References

Shimizu C, et al. Characterization of a white bass (Morone chrysops) embryonic cell line with epithelial features. In Vitro Cell. Dev. Biol. Anim. 39: 29-35, 2003. PubMed: 12892524

Shimizu C, et al. Characterization of a white bass (Morone chrysops) embryonic cell line with epithelial features. In Vitro Cell. Dev. Biol. Anim. 39: 29-35, 2003. PubMed: 12892524

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Shimizu C, et al. Characterization of a white bass (Morone chrysops) embryonic cell line with epithelial features. In Vitro Cell. Dev. Biol. Anim. 39: 29-35, 2003. PubMed: 12892524

Shimizu C, et al. Characterization of a white bass (Morone chrysops) embryonic cell line with epithelial features. In Vitro Cell. Dev. Biol. Anim. 39: 29-35, 2003. PubMed: 12892524