IP-1B (ATCC® CRL-2162)

Organism: Mus musculus, mouse  /  Cell Type: endothelial, SV40 transformed  / 

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Organism Mus musculus, mouse
Cell Type endothelial, SV40 transformed
Product Format frozen
Morphology epithelial
Culture Properties adherent
Biosafety Level 2 Cells contain polyomavirus DNA sequences
Age adult
Gender male
Strain C3H/HeJ
Applications
The IP-1B cell line was derived from an ascites tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161).
Like 3B-11 (see ATCC CRL-2160), which was cloned from a tumor that developed in a different mouse, IP-1B is resistant to lysis by activated macrophages as measured in the chromium release assay.
This clone retains the ability to differentiate on a synthetic basement-like membrane.
Derivation
The IP-1B cell line was derived from an ascites tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161).
Like 3B-11 (see ATCC CRL-2160), which was cloned from a tumor that developed in a different mouse, IP-1B is resistant to lysis by activated macrophages as measured in the chromium release assay.
Clinical Data
male
Antigen Expression
H-2 K; VCAM
Genes Expressed
H-2 K; VCAM
Tumorigenic Yes
Effects
Yes, the cells induced spindle tumors in nude mice with some of the histopathologic characteristics of human Kaposi Sarcoma after a shortened latency period of approximately 2 weeks
Comments
The IP-1B cell line was derived from an ascites tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161).
The line was cloned in 1991 by limiting dilution.
Like 3B-11 (see ATCC CRL-2160), which was cloned from a tumor that developed in a different mouse, IP-1B is resistant to lysis by activated macrophages as measured in the chromium release assay.
This clone retains the ability to differentiate on a synthetic basement-like membrane.
The cells express the cell surface major histocompatibility complex class I antigen, H-2 k, of the parental cell line, and express VCAM (vascular cell adhesion molecule).
The cells stain positively for SV40 T antigen.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml), and let the culture sit at room temperature until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Cryopreservation
Culture medium, 95%; DMSO, 5%
Name of Depositor KA O'Connell
References

O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170

O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612

O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170

O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612

O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. PubMed: 1928299