BCE C/D-1b (ATCC® CRL-2048)

Organism: Bos taurus, cow  /  Cell Type: endothelial  /  Tissue: eye, cornea  / 

Organism Bos taurus, cow
Tissue eye, cornea
Cell Type endothelial
Product Format frozen
Morphology endothelial
Culture Properties adherent
Biosafety Level 1
Age adult
Gender male
Applications
The cells produce a extracellular matrix that can be used to support the growth of other cells including rat neuronal cells and astrocytes.
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
This line was established from explants of normal adult bovine corneas.
Genes Expressed
extracellular matrix (ECM) proteins
Cellular Products
extracellular matrix (ECM) proteins
Comments

The reported population doubling time decreases with increasing passages from approximately 55 hours at passage 70 to 30 hours at passage 90.

The cells will grow well (but at a slower rate) in medium containing decreased amounts of serum (5% bovine calf serum, 2.5% fetal bovine serum).

Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing Remove medium, add fresh trypsin (0.25%) - EDTA (0.03%) solution, rinse and remove. Add fresh trypsin solution (1 to 2 mL) and allow flasks to remain at room temperature (or incubate at 37°C) until cells detach. Add fresh medium, aspirate and dispense into new culture flasks.
Subcultivation ratio: 1:2 to 1:4
Medium Renewal: 2 to 3 times per week
Culture Conditions
Temperature: 37°C
Name of Depositor AH Nicholas, C Bethea
Passage History
The reported population doubling time decreases with increasing passages from approximately 55 hours at passage 70 to 30 hours at passage 90.
References

Gospodarowicz D, et al. Stimulation of corneal endothelial cell proliferations in vitro by fibroblast and epidermal growth factors. Exp. Eye Res. 25: 75-89, 1977. PubMed: 302215

Bethea CL, Kozak SL. Effect of extracellular matrix on PC 12 cell shape and dopamine processing. Mol. Cell. Endocrinol. 37: 319-327, 1984. PubMed: 6500171

Basic Documentation
Other Documentation
References

Gospodarowicz D, et al. Stimulation of corneal endothelial cell proliferations in vitro by fibroblast and epidermal growth factors. Exp. Eye Res. 25: 75-89, 1977. PubMed: 302215

Bethea CL, Kozak SL. Effect of extracellular matrix on PC 12 cell shape and dopamine processing. Mol. Cell. Endocrinol. 37: 319-327, 1984. PubMed: 6500171