KG-1 (ATCC® CRL-8031)

Organism: Homo sapiens, human  /  Tissue: bone marrow  /  Disease: acute myelogenous leukemia

Permits and Restrictions

View Permits

Organism Homo sapiens, human
Tissue bone marrow
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Disease acute myelogenous leukemia
Age 59 years
Gender male
Ethnicity Caucasian
Applications Produces high levels of human leukocyte interferon upon induction by paramyxoviridae species.
Storage Conditions liquid nitrogen vapor phase
Clinical Data
male
Caucasian
59 years
Genes Expressed
human leukocyte interferon
Comments
Responsive to colony stimulating activity (CSA).
See also ATCC CCL-246.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 X 105 viable cells/mL.
Interval: Maintain between 2 X 105 and 1 X 106 viable cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Culture Conditions
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C
STR Profile
Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19
Name of Depositor Genentech, Inc.
References

Koeffler HP, Golde DW. Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity. Science 200: 1153-1154, 1978. PubMed: 306682

Goeddel DV. Hybrid human leukocyte interferons. US Patent 4,678,751 dated Jul 7 1987

Cross References

Nucleotide (GenBank) : E00473 Human leukocyte interferon A (LeIFA) gene and its flanking sequence.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Koeffler HP, Golde DW. Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity. Science 200: 1153-1154, 1978. PubMed: 306682

Goeddel DV. Hybrid human leukocyte interferons. US Patent 4,678,751 dated Jul 7 1987