MM.1R (ATCC® CRL-2975)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast  /  Tissue: peripheral blood  /  Disease: multiple myeloma

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Organism Homo sapiens, human
Tissue peripheral blood
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties mixed, suspension and lightly attached cells
Biosafety Level 1
Disease multiple myeloma
Age 42 years
Gender female
Ethnicity Black
Applications
MM.1R is resistant to dexamethasone.
Storage Conditions liquid nitrogen vapor phase
Images
Derivation
The parent cell line, MM.1, was established from peripheral blood of a multiple myeloma patient who had become resistant to steroid-based therapy.
Clinical Data
female
42 years
Black
Antigen Expression
CD25-, CD38+, CD52+, CD59+
Receptor Expression
glucocorticoid receptor, not expressed
Genes Expressed
lambda-light chain immunoglobulin
Cellular Products
lambda-light chain immunoglobulin
Comments
MM.1R is resistant to dexamethasone. The closely related cell line, MM.1S (ATCC CRL-2974) was also isolated from MM.1, but is sensitive to dexamethasone.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

This is a mixed cell culture; cells grow both as a lightly attached monolayer and in suspension. Cultures can be maintained by adding fresh medium. Alternatively, subcultures can be prepared by scraping the adherent cells into the medium containing the floating cells, collecting the cells by centrifugation, resuspending the cell pellet in fresh medium and dispensing into new flasks.

Note: Subculture cells before they reach confluence; cells in over-confluent cultures begin to form rosettes with necrotic centers.
 

Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended.
Medium renewal: Every 2 to 3 days.
Cryopreservation
Freeze medium: complete growth medium, 90%; DMSO, 10%
liquid nitrogen vapor phase
Culture Conditions
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X
CSF1PO: 9, 14
D13S317: 13
D16S539: 12
D5S818: 8, 12
D7S820: 10, 11
THO1: 7, 8
TPOX: 8
vWA: 15, 17
Population Doubling Time approximately 72 hours
Name of Depositor S. Rosen
Year of Origin 1990
References

Goldman-Leikin RE, et al. Characterization of a novel myeloma cell line, MM.1. J. Lab. Clin. Med. 113(3):335-345, 1989. PubMed: 2926241

Greenstein S, et al. Characterization of the MM.1 human multiple myeloma (MM) cell lines: A model system to elucidate the characteristics, behavior, and signaling of steroid-sensitive and -resistant MM cells. Exp. Hematol.: 31:271-282, 2003. PubMed; 12691914

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Goldman-Leikin RE, et al. Characterization of a novel myeloma cell line, MM.1. J. Lab. Clin. Med. 113(3):335-345, 1989. PubMed: 2926241

Greenstein S, et al. Characterization of the MM.1 human multiple myeloma (MM) cell lines: A model system to elucidate the characteristics, behavior, and signaling of steroid-sensitive and -resistant MM cells. Exp. Hematol.: 31:271-282, 2003. PubMed; 12691914