Mo-B (ATCC® CCL-245)

Organism: Homo sapiens, human  /  Cell Type: B lymphoblast  /  Disease: hairy cell leukemia

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Organism Homo sapiens, human
Cell Type B lymphoblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2 Cells Contain RETROVIRUS
Disease hairy cell leukemia
Age 33 years
Gender male
Ethnicity Caucasian
Applications
transfection host
Clinical Data
male
Caucasian
33 years
Genes Expressed
cells produce infectious HTLV II
Cellular Products
cells produce infectious HTLV II
Comments
The cells produce infectious human T cell leukemia virus and must be handled as a potential biohazard under P-2 conditions. The line is EBNA positive (EBNA+).
Complete Growth Medium Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Subculturing
Protocol: The cells are subcultured every 7 days by centrifugation .For optimum results do not culture below 8 X 10(5 ) cells/ml or above 2 X 10(6) cells/ml.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: none
STR Profile
Amelogenin: X,Y
CSF1PO: 10,13
D13S317: 9,13
D16S539: 11
D5S818: 11,12
D7S820: 9,12
THO1: 9.3
TPOX: 8,11
vWA: 17,18
Isoenzymes
ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1
PGM3, 0
Name of Depositor DW Golde
References

Chen IS, et al. Human T-cell leukemia virus type-II transforms normal human lymphocytes. Proc. Natl. Acad. Sci. USA 80: 7006-7009, 1983. PubMed: 6316341

Saxon A, et al. T-lymphocyte variant of hairy-cell leukemia. Ann. Intern. Med. 88: 323-326, 1978. PubMed: 305224

Chen IS, et al. Molecular characterization of genome of a novel human T-cell leukaemia virus. Nature 305: 502-505, 1983. PubMed: 6312323

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
References

Chen IS, et al. Human T-cell leukemia virus type-II transforms normal human lymphocytes. Proc. Natl. Acad. Sci. USA 80: 7006-7009, 1983. PubMed: 6316341

Saxon A, et al. T-lymphocyte variant of hairy-cell leukemia. Ann. Intern. Med. 88: 323-326, 1978. PubMed: 305224

Chen IS, et al. Molecular characterization of genome of a novel human T-cell leukaemia virus. Nature 305: 502-505, 1983. PubMed: 6312323