2BD4E4 K99 (ATCC® HB-8178)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Strain

Strain:  BALB/c (B cell); BALB/c (myeloma)

Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against Escherichia coli K99 pilus
Cellular Products
immunoglobulin; monoclonal antibody; against Escherichia coli K99 pilus
Comments
Animals were immunized with E. coli K99 pilus.
Spleen cells were fused with NS-1 myeloma cells.
Complete Growth Medium The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 15%

  • This medium is formulated for use with a 5% CO2 in air atmosphere. (Standard DMEM formulations contain 3.7 g/L sodium bicarbonate and a 10% CO2 in air atmosphere is then recommended).
    Subculturing
    Medium Renewal: Every 3 to 4 days
    Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.
    Cryopreservation
    culture medium 95%; DMSO, 5%
    Culture Conditions
    Temperature: 37.0°C
    Isotype IgG1; kappa light chain
    Name of Depositor Molecular Genetics, Inc.
    U.S. Patent Number
    Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
    References

    Chan R, et al. Use of specific antibody to demonstrate glycocalyx, K99 pili and the spatial relationships of K99 & enterotoxigenic Escherichia coli in the ileum of colostrum-fed calves. Infect. Immun. 37: 1170-1180, 1982. PubMed: 6127313

    Sadowski PL. Production of monoclonal antibodies against bacterial adhesins. US Patent 4,443,549 dated Apr 17 1984

    Basic Documentation
    References

    Chan R, et al. Use of specific antibody to demonstrate glycocalyx, K99 pili and the spatial relationships of K99 & enterotoxigenic Escherichia coli in the ileum of colostrum-fed calves. Infect. Immun. 37: 1170-1180, 1982. PubMed: 6127313

    Sadowski PL. Production of monoclonal antibodies against bacterial adhesins. US Patent 4,443,549 dated Apr 17 1984