Chlamydia trachomatis (ATCC® VR-886)

Classification: Chlamydiaceae, Chlamydia  /  Product Format: frozen 1 mL per vial

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Classification Chlamydiaceae, Chlamydia
Deposited As Chlamydia trachomatis (Busacca) Rake
Agent Chlamydia trachomatis
Strain Trachoma type J strain UW-36/Cx
Biosafety Level 2
Product Format frozen 1 mL per vial
Storage Conditions -70°C or colder
Comments FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.
Antigenically related but distinct from type C.
Disrupt cells of seed stock using glass beads and vortex. Infect shell vial monolayer with 0.1 mL of diluted seed stock. contrifuge at 2000X for 1 hr. Feed with medium containing cycloheximide.
This preparation has tested negative for Mycoplasma by culture; the PCR test results are ambiguous.
Antigenically related but distinct from type C.
Effect on Host
CPE, intracellular inclusion bodies
Recommended Host
McCoy cells (ATCC CRL-1696)
Growth Conditions
Temperature: 36°C
Recommendations for Infection: For best results cells should be 24 to 48 hours old and 90-100% confluent.
Incubation: 3 days, a 5% CO2 in air atmosphere is recommended
Effect on Host
CPE, intracellular inclusion bodies
Name of Depositor SP Wang, CDC
Source Human cervix, cervicitis, Seattle, 1971
Year of Origin 1971
References

Wang S, Grayston JT. Chlamydia trachomatis immunotype J. J. Immunol. 115: 1711-1716, 1975. PubMed: 810514

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation