Simian virus 40 (ATCC® VRMC-6)

Organism: Simian virus 40, Simian virus 40  /  Clone Type: Clone  /  Depositors: JA Lednicky, JS Butel

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Designations pUCSV-VA45-54-2Ea [molecular clone of Simian virus 40, SV-40]
GenBank Number


Species Simian virus 40, Simian virus 40
Depositors JA Lednicky, JS Butel
contains sequence
Construct size (kb): 7.949999809265137
Insert lengths(kb): 5.263999938964844
Tissue: uninoculated grivet monkey cells
Gene product: [SV-VA45-54-2Ea]
Insert Size (kb) 5.264
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2
Insert contains following restriction sites (from 5'end): BamHI-0.771,7.179; EcoRI-2.686,5.264; KpnI-3.786,4.164; SalI-7.950; SfiI-7.950; XhoI-uncut. Plasmid size: 7950bp (2686bp pUC-19vector+ 5264bp SV40-VA45-54-2 insert).
This variant of SV-40-VA45-54 has an non-archetypal viral regulatory region and was verified by lipofection of EcoRI-linearized plasmid pUCSV-VA45-54-2Ea DNA into ATCC CV-1 cells by the contributor, followed by DNA sequence analysis of the virus stock.
Classification Polyomaviridae, Polyomavirus

Lednicky JA, Butel JS. Tissue culture adaptation of natural isolates of simian virus 40: changes occur in viral regulatory region but not in carboxy-terminal domain of large T-antigen. J. Gen. Virol. 78: 1697-1705, 1997. PubMed: 9225047

Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955

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