Simian virus 40 (ATCC® VRMC-6)

Classification: Polyomaviridae, Polyomavirus  /  Product Format: frozen

Classification Polyomaviridae, Polyomavirus
Agent Simian virus 40
Strain pUCSV-VA45-54-2Ea [molecular clone of Simian virus 40, SV-40]
Common Name Simian virus 40
Applications
contains sequence
Biosafety Level 2
Product Format frozen
Comments
Insert contains following restriction sites (from 5'end): BamHI-0.771,7.179; EcoRI-2.686,5.264; KpnI-3.786,4.164; SalI-7.950; SfiI-7.950; XhoI-uncut. Plasmid size: 7950bp (2686bp pUC-19vector+ 5264bp SV40-VA45-54-2 insert).
This variant of SV-40-VA45-54 has an non-archetypal viral regulatory region and was verified by lipofection of EcoRI-linearized plasmid pUCSV-VA45-54-2Ea DNA into ATCC CV-1 cells by the contributor, followed by DNA sequence analysis of the virus stock.
Growth Medium ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Recommended Host
E. coli
Growth Conditions
Temperature: 37.0°C
Name of Depositor JA Lednicky, JS Butel
Special Collection NCRR Contract
References

Lednicky JA, Butel JS. Tissue culture adaptation of natural isolates of simian virus 40: changes occur in viral regulatory region but not in carboxy-terminal domain of large T-antigen. J. Gen. Virol. 78: 1697-1705, 1997. PubMed: 9225047

Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955