pUCSV40-CPC/MEN-1 [molecular clone of Simian virus 40, SV-40] (ATCC^® VRMC-4^™)

Organism: Simian virus 40, Simian virus 40  /  Tissue: Clone  /  Depositors: JA Lednicky, JS Butel

Designations	pUCSV40-CPC/MEN-1 [molecular clone of Simian virus 40, SV-40]
GenBank Number	AF156108
Species	Simian virus 40, Simian virus 40
Depositors	JA Lednicky, JS Butel
Isolation	Contains a full-length genome of SV-40 strain CPC/MEN cloned into the EcoRI site of pUC-19. SV40-CPC/MEN was isolated and cloned from virus stock derived from choroid plexus carcinoma DNA sample 12 (from a 4 yr old child).
Applications	contains sequence
Vector	Construct size (kb): 7.866000175476074 DESCRIPTION OF VECTOR COMPONENT         Type of vector:  E. coli         Name of vector: pUC-19         Intact vector size (kb):  2.686 kb
Insert	DNA: cDNA Insert lengths(kb): 5.179999828338623 Tissue: choroid plexus carcinoma Gene product: [SV40-CPC/MEN-1]
Insert Size (kb)	5.180
Media	ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level	2
Comments	It has an archetypal viral regulatory region and was tested by lipofection of EcoRI-linearized plasmid DNA into TC7 cells, with cells showing subsequent production of typical SV-40 CPE. Lipofection into TC7 and Butel lab CV-1 cells was verified. Contains a full-length genome of SV-40 strain CPC/MEN cloned into the EcoRI site of pUC-19. SV40-CPC/MEN was isolated and cloned from virus stock derived from choroid plexus carcinoma DNA sample 12 (from a 4 yr old child).
Classification	Polyomaviridae, Polyomavirus
References	Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955 Lednicky JA, et al. Natural simian virus 40 strains are present in human choroid plexus and ependymoma tumors. Virology 212: 710-717, 1995. PubMed: 7571441

Product Sheet	Product Sheet