Simian virus 40 (ATCC® VRMC-35)

Classification: Polyomaviridae, Polyomavirus  /  Product Format: frozen

Classification Polyomaviridae, Polyomavirus
Agent Simian virus 40
Strain pUCSV40-777-5 [molecular clone of Simian virus 40, SV-40]
Common Name Simian virus 40
Applications
contains sequence
Biosafety Level 2
Isolation
Description of cloned agent:
Product Format frozen
Comments
The insert contains the following restriction sites (kb): BamHI--4.549, 3.417; EcoRI--5.215, 2.686; KpnI--6.365, 1.591; SalI--7.956; SfiI--7.956; XhoI--uncut
Description of cloned agent:
Name: SV40
Classification: Polyomavirus
Plasmid copy#: High-copy number (pUC19 has a ColEI ori)
Viable virus clone. Verified by lipofection of EcoRI-linearized plasmid DNA into ATCC CV-1 cells (passage# 40) by J. Lednicky (lipofection date: 12 Oct. 2000).
The viral regulatory region of this construct is complex (non-archetypal) but distinct from the complex regulatory region of SV40-777-5. Plasmid size: 7.956 kb [2.686 kb (pUC19 vector) + 5.270 kb (SV40-777-5 insert)]
Name of Depositor JA Lednicky
Special Collection NCRR Contract