Simian virus 40 (ATCC® VRMC-3)

Classification: Polyomaviridae, Polyomavirus  /  Product Format: frozen

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Classification Polyomaviridae, Polyomavirus
Agent Simian virus 40
Strain pUCSV40-B2E [molecular clone of Simian virus 40, SV-40]
Common Name Simian virus 40
Applications
contains sequence
Biosafety Level 2
Isolation
Contains a full-length genome of SV40-B2 cloned into pUC-19 at the EcoRI site. SV40-B2 was isolated and cloned from a low-passage stock of SV40-Baylor that had been isolated from a contaminated poliovaccine.
Product Format frozen
Shipping Information  Frozen glycerol stock of Escherichia coli containing the plasmid.
Storage Conditions Store unopened frozen vial at -80°C.
Comments
This variant of SV-40-Baylor has a non-archetypal viral regulatory region and is the commonly studied version of SV-40-Baylor. Faster growing than SV-40-B1 in BSC-1, Vero, CV-1, and TC-7 cells.
Contains a full-length genome of SV40-B2 cloned into pUC-19 at the EcoRI site. SV40-B2 was isolated and cloned from a low-passage stock of SV40-Baylor that had been isolated from a contaminated poliovaccine.
Growth Medium ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Recommended Host
E. coli
Growth Conditions
Temperature: 37.0°C
Name of Depositor JA Lednicky, JS Butel
Special Collection NCRR Contract
References

Lednicky JA, Butel JS. Tissue culture adaptation of natural isolates of simian virus 40: changes occur in viral regulatory region but not in carboxy-terminal domain of large T-antigen. J. Gen. Virol. 78: 1697-1705, 1997. PubMed: 9225047

Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation