Simian virus 40 (ATCC® VRMC-2™)

Classification: Polyomaviridae, Polyomavirus  /  Product Format: frozen

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Classification Polyomaviridae, Polyomavirus
Agent Simian virus 40
Strain pUCSV40-B1E [molecular clone of Simian virus 40, SV-40]
Common Name Simian virus 40
Applications
contains sequence
Biosafety Level 2
Isolation
Contains a full-length genome of SV40-B1 cloned into pUC-19 at the EcoRI site. SV40-B1 was isolated and cloned from a low-passage stock of SV40-Baylor that had been isolated from a contaminated poliovaccine. The viral regulatory region is archetypal.
Product Format frozen
Shipping Information Distributed: Frozen DNA in TE buffer
Comments
This variant of SV40-Baylor has an archetypal viral regulatory region and can be used in viral growth studies for comparison with SV40-B2, the commonly used laboratory variant of SV40-Baylor.
Contains a full-length genome of SV40-B1 cloned into pUC-19 at the EcoRI site. SV40-B1 was isolated and cloned from a low-passage stock of SV40-Baylor that had been isolated from a contaminated poliovaccine. The viral regulatory region is archetypal.
Effect on Host
This variant of SV40-Baylor has an archetypal viral regulatory region and can be used in viral growth studies for comparison with SV40-B2, the commonly used laboratory variant of SV40-Baylor.
Growth Medium ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Recommended Host
E. coli
Growth Conditions
Temperature: 37.0°C
Effect on Host
This variant of SV40-Baylor has an archetypal viral regulatory region and can be used in viral growth studies for comparison with SV40-B2, the commonly used laboratory variant of SV40-Baylor.
Name of Depositor JA Lednicky, JS Butel
Special Collection NCRR Contract
References

Lednicky JA, Butel JS. Tissue culture adaptation of natural isolates of simian virus 40: changes occur in viral regulatory region but not in carboxy-terminal domain of large T-antigen. J. Gen. Virol. 78: 1697-1705, 1997. PubMed: 9225047

Stewart AR, et al. Sequence analyses of human tumor-associated SV40 DNAs and SV40 viral isolates from monkeys and humans. J. Neurovirol. 4: 182-193, 1998. PubMed: 9584955

Cross References

Nucleotide (GenBank)
AF155359
Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS