pUCSV40-T302-1 [molecular clone of Simian virus 40, SV-40] (ATCC® VRMC-14)

Organism: Simian virus 40, Simian virus 40  /  Clone Type: Clone  /  Depositors: JA Lednicky, JS Butel

Permits and Restrictions

View Permits

Designations pUCSV40-T302-1 [molecular clone of Simian virus 40, SV-40]
Species Simian virus 40, Simian virus 40
Depositors JA Lednicky, JS Butel
Origin
SV40-T302-1 was isolated in CV-1 cells (ATCC CCL-70) from the brain of an SIV-infected juvenile Rhesus monkey. This is a clone of a unique brain isolate of SV40 (slowest growing SV40 natural isolate encountered at time of deposit.)
Isolation
SV40-T302-1 was isolated in CV-1 cells (ATCC CCL-70) from the brain of an SIV-infected juvenile Rhesus monkey. This is a clone of a unique brain isolate of SV40 (slowest growing SV40 natural isolate encountered at time of deposit.)
Applications
contains sequence
Vector
Construct size (kb): 7.842000007629395

DESCRIPTION OF VECTOR COMPONENT

        Type of vector:  E. coli

        Name of vector:  pUC-19

        Intact vector size (kb):  2.686 kb

Insert
DNA: genomic
Insert lengths(kb): 5.156000137329102
Tissue: Rhesus monkey brain
Insert Size (kb) 5.156
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2
Comments
Description of cloned agent:
Name: SV40
Strain: T302-1
Classification: Papovavirus
Full length clone of SV40 strain T302
Brain tissue was minced in PBS, freeze-thawed three times to lyse the cells, then passed through a 0.45 mm filter. The filtrate was used to inoculate ATCC CV-1 cells (passage 34). The viral regulatory region of this construct is archetypal.
Classification Polyomaviridae, Polyomavirus
References

Lednicky JA, et al. Natural isolates of simian virus 40 from immunocompromised monkeys display extensive genetic heterogeneity: new implications for polyomavirus disease. J. Virol. 72: 3980-3990, 1998. PubMed: 9557685

Product Sheet
Product Sheet
Product Sheet