Simian virus 40 (ATCC® VRMC-13)

Organism: Simian virus 40, Simian virus 40  /  Clone Type: Clone  /  Depositors: JA Lednicky, JS Butel

Permits and Restrictions

View Permits

Designations pUCSV40-2895-2 [molecular clone of Simian virus 40, SV-40]
Species Simian virus 40, Simian virus 40
Depositors JA Lednicky, JS Butel
Origin
SV40-A2895-2 was isolated from a virus-cell lysate of CV-1 cells (ATCC CCL-70) inoculated with the A2895 strain of SV40 (ATCC VR-305, Lot 7DR).
Isolation
SV40-A2895-2 was isolated from a virus-cell lysate of CV-1 cells (ATCC CCL-70) inoculated with the A2895 strain of SV40 (ATCC VR-305, Lot 7DR).
Applications
contains sequence
Vector
Construct size (kb): 7.927999973297119

DESCRIPTION OF VECTOR COMPONENT

        Type of vector:  E. coli

        Name of vector:  pUC-19

        Intact vector size (kb):  2.686 kb

Insert
DNA: genomic
Insert lengths(kb): 5.242000102996826
Insert Size (kb) 5.242
Media Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2
Comments
Description of cloned agent:
Name: SV40
Strain: A2895 regulatory region variant 2
Classification: Papovavirus
Full length clone of SV40 strain A2895
The viral regulatory region of this construct is non-archetypal (complex), but different (larger than) the SV40-A2895-1 (VRMC-12) regulatory region.
Verified infectious by lipofection of EcoR1-linearized plasmid DNA into CV-1 cells (ATCC CCL-70) by J. Lednicky.
Classification Polyomaviridae, Polyomavirus
Product Sheet
Product Sheet
Product Sheet