Simian virus 40 (ATCC® VRMC-13)

Organism: Simian virus 40, Simian virus 40  /  Clone Type: Clone  /  Depositors: JA Lednicky, JS Butel

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Designations pUCSV40-2895-2 [molecular clone of Simian virus 40, SV-40]
Species Simian virus 40, Simian virus 40
Depositors JA Lednicky, JS Butel
Origin
SV40-A2895-2 was isolated from a virus-cell lysate of CV-1 cells (ATCC CCL-70) inoculated with the A2895 strain of SV40 (ATCC VR-305, Lot 7DR).
Isolation
SV40-A2895-2 was isolated from a virus-cell lysate of CV-1 cells (ATCC CCL-70) inoculated with the A2895 strain of SV40 (ATCC VR-305, Lot 7DR).
Applications
contains sequence
Vector
Construct size (kb): 7.927999973297119

DESCRIPTION OF VECTOR COMPONENT

        Type of vector:  E. coli

        Name of vector:  pUC-19

        Intact vector size (kb):  2.686 kb

Insert
DNA: genomic
Insert lengths(kb): 5.242000102996826
Insert Size (kb) 5.242
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 2

Biosafety  classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Description of cloned agent:
Name: SV40
Strain: A2895 regulatory region variant 2
Classification: Papovavirus
Full length clone of SV40 strain A2895
The viral regulatory region of this construct is non-archetypal (complex), but different (larger than) the SV40-A2895-1 (VRMC-12) regulatory region.
Verified infectious by lipofection of EcoR1-linearized plasmid DNA into CV-1 cells (ATCC CCL-70) by J. Lednicky.
Classification Polyomaviridae, Polyomavirus