BK polyomavirus (ATCC® VR-837™) Classification: Polyomaviridae, Polyomavirus / Product Format: 1 mL per vial General Information Characteristics Culture Method Specifications History Documentation Print Email Share Share this product Facebook Twitter Google+ Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Classification Polyomaviridae, Polyomavirus Agent BK polyomavirus Biosafety Level 2 Product Format 1 mL per vial Storage Conditions -70°C or colder Comments Hemagglutination of 0.5% GPRBC. Virus MUST be freeze-thaw cycled 6 times for greatest infectivity. Effect on Host CPE, monolayer degeneration and some cell rounding Recommended Host HEL 299 (ATCC® CCL-137™) Growth Conditions Temperature: 37°C Recommendations For Infection: For best results, cells should be 24 to 48 hours old and 70-90% confluent (not 100% confluent). Incubation: 14-23 days, a 5% CO2 in air atmosphere is recommended Effect on Host CPE, monolayer degeneration and some cell rounding Name of Depositor G di Mayorca Source Urine of a kidney transplant patient, London, 1970 References Gardner SD, et al. New human papovavirus (B.K.) isolated from urine after renal transplantation. Lancet 1: 1253-1257, 1971. PubMed: 4104714 Permits These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment. Basic Documentation Product Sheet Certificate of Analysis MSDS