DNA from HCMV strain AD-169 [ATCC VR-538] (ATCC^® VR-538D^™)

DNA from HCMV strain AD-169 [ATCC VR-538] ATCC^® VR-538D^™

frozen Total DNA from infected cells, 100 µL per vial

For-Profit: $290.00 Non-Profit: $290.00

Qty:

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Product Format	frozen Total DNA from infected cells, 100 µL per vial
Storage Conditions	-70°C or colder
Applications	It is appropriate for use in polymerase chain reaction (PCR) for viral gene products and other molecular virology applications.
Comments	This product is a preparation of viral nucleic acids, including virus genomic DNA, in total DNA from MRC-5 cells (ATCC CCL-171) infected with Human herpesvirus 5 (HCMV) strain AD-169 (VR-538). The product is supplied at 100 ul per vial, shipped frozen. It is appropriate for use in polymerase chain reaction (PCR) for viral gene products and other molecular virology applications. Strain AD-169 has been completely sequenced. Genbank: X17403
Biosafety Level	1

Quality Control Specifications	Store at -20°C or colder. -80°C or vapor-phase liquid nitrogen (-196°C) is preferred for long-term storage (more than one month). Concentration is verified as > 1 µg/mL DNA by Picogreen^® assay. Purity is confirmed by OD₂₆₀/OD₂₈₀ ratio (> 1.7) of bulk DNA prior to dilution for product filling. Integrity is inferred from observation of high molecular weight cellular DNA following electrophoresis 20 uL of product on a 0.8% agarose gel, visualized by ethidium bromide staining. Functional activity is demonstrated by PCR amplification of a 500-3,000 bp amplicon using virus-specific primers. Identity is confirmed by sequencing of ~300 base pairs of the PCR amplicon. Absence of infectious virus is verified by incubation of 10% of each lot with susceptible host cells under appropriate conditions for a period of 7-14 days.

Quality Control Specifications

Store at -20°C or colder. -80°C or vapor-phase liquid nitrogen (-196°C) is preferred for long-term storage (more than one month).
Concentration is verified as > 1 µg/mL DNA by Picogreen^® assay.
Purity is confirmed by OD₂₆₀/OD₂₈₀ ratio (> 1.7) of bulk DNA prior to dilution for product filling.
Integrity is inferred from observation of high molecular weight cellular DNA following electrophoresis 20 uL of product on a 0.8% agarose gel, visualized by ethidium bromide staining.
Functional activity is demonstrated by PCR amplification of a 500-3,000 bp amplicon using virus-specific primers.
Identity is confirmed by sequencing of ~300 base pairs of the PCR amplicon.
Absence of infectious virus is verified by incubation of 10% of each lot with susceptible host cells under appropriate conditions for a period of 7-14 days.

Permits	These permits may be required for shipping this product: Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS