Chlamydophila pneumoniae (ATCC® VR-2282)

Classification: Chlamydiaceae, Chlamydophila  /  Product Format: frozen

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Classification Chlamydiaceae, Chlamydophila
Agent Chlamydophila pneumoniae
Strain TWAR strain TW-183
Applications
Respiratory research
Positive for Mycoplasma Yes
Biosafety Level 2
Isolation
conjunctiva of a child, Taiwan, 1965
Product Format frozen
Storage Conditions -70°C or colder
Comments
Note that activities with high potential for aerosol production require BSL 3 facilities and practices.
For a time, prior to the acceptance of the C. pneumoniae strains as a separate species, the strains were refered to as TWAR, after the isolates TW-183 and AR-39. AR-39 is available as ATCC #53592.
The inclusions are iodine stain negative (contain no glycogen).
Suggested protocol for propagation: Add glass beads and vortex preparation to disrupt cells. Infect monolayer with disrupted material. Centrifuge at 3000 x rpm (750 x g) for 1 hour.
Feed with fresh growth medium containing FBS prescreened for Chlamydia antibodies and 1-2 ug/mL cycloheximide. Incubate at 37C for 72 hours.
Infection of TC is enhanced by subconfluent monolayer of cells.
This preparation has tested positive for Mycoplasma contamination.
The inclusions are iodine stain negative (contain no glycogen). The TWAR strains (now C. pneumoniae) of Chlamydia are named after TW183 and AR39.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies
Recommended Host
HEp-2 cells (ATCC CCL-23)
chicken embryo; HeLa 229 cells (ATCC CCL-2.1)
Growth Conditions
Atmosphere: 5% CO2 in air recommended
Temperature: 35°C
Incubation: 3 days at 35°C
Cryopreservation
Storage temperature: -70C or lower
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific monoclonal antibodies
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies
Name of Depositor G Galloway, T Grayston
Special Collection NCRR Contract
Source
conjunctiva of a child, Taiwan, 1965
Year of Origin 1965
References

Grayston JT, et al. Chlamydia pneumoniae sp. nov. for Chlamydia sp. strain TWAR. Int. J. Syst. Bacteriol. 39: 88-90, 1989.

Kuo CC, Grayston JT. Chlamydia spp. strain TWAR: A newly recognized organism associated with atypical pneumonia and other respiratory infections. Clin. Microbiol. Newsletter 10: 137-140, 1988.

Everett KD, et al. Emended description of the order Chlamydiales, proposal of Parachlamydiaceae fam. nov. and Simkaniaceae fam. nov., each containing one monotypic genus, revised taxonomy of the family Chlamydiaceae, including a new genus and five new species, and standards for the identification of organisms. Int. J. Syst. Bacteriol. 49: 415-440, 1999. PubMed: 10319462

type strain

Cross References

Nucleotide (GenBank) : AJ012174 Chlamydophila pneumoniae partial rnpB gene for RNase P RNA subunit.

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation