Recommendations For Infection: For best results, plate cells 24-48 hours in advance. Infect at 80-90% confluent (not 100% confluent) using a multiplicity of infection (MOI) of 0.01 to 1.0. Incubate at 35°C in a humidified, 5% CO2 atmosphere for 2-4 days, until CPE are well advanced in 100% of the culture. In TC, ATCC® VR-1680 should be grown in serum free media that contains 1 ug/mL TPCK-treated trypsin, 0.125% BSA and 1% HEPES (1 molar stock).
Incubation: 2-4 days