Saccharomyces cerevisiae Hansen, teleomorph (ATCC® MYA-4940)

Strain Designations: YVH10  /  Product Format: frozen

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Deposited As Saccharomyces cerevisiae
Strain Designations YVH10
Application Used in the yeast display method to isolate novel proteins based on various biological functions
Biosafety Level 1
Product Format frozen
Type Strain no
Genotype MATalpha PDI1::GAPDH-PDI1::LEU2 ura3- 52 trp1 leu2-delta200 his3-delta200 pep4::HIS3 prb11.6R can1 GAL
Preceptrol® no
Comments YVH10 (Ura-, Trp-) is BJ5464 and is MAT-alpha. The other markers are: ura3-52 (a Ty element insertion with no detectable background reversion frequency), trp1 (an amber point mutation), leu2-delta200, his3-delta200, pep4:HIS3, prbd1.6R, can1, GAL. YVH10 has the yeast PDI gene integrated in tandem with the endogenous copy and is under the constitutive glyceraldehydes (GADPH) promoter with Leu2 as a selectable marker.
Morphology On YM medium at 30°C for 2 days, colonies creamy white to dingy white, butyrous, smooth, slightly raised, margin entire. Cells globose to subglobose, hyaline, smooth, contain oil droplets, 4-6 X 3-5 µm. Pseudohyphae not observed.
Sequenced Data
Not available.
Morphology On YM medium at 30°C for 2 days, colonies creamy white to dingy white, butyrous, smooth, slightly raised, margin entire. Cells globose to subglobose, hyaline, smooth, contain oil droplets, 4-6 X 3-5 µm. Pseudohyphae not observed.
Name of Depositor KD Wittrup
Chain of Custody ATCC <-- KD Wittrup
Isolation From parent strain BJ5464
References

Robinson AS, Hines V, Wittrup KD. Protein disulfide isomerase overexpression increases secretion of foreign proteins in Saccharomyces cerevisiae. Biotechnology 12(4): 381-384, 1994. PubMed: 7764684

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These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
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