Geomyces destructans Blehert et Gargas ATCC ® MYA-4855™

Permits	These permits may be required for shipping this product: Customer Acceptance of Responsibility, ATCC Form 62 required for distribution. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Strain Designations	20631-21
Application	Emerging infectious disease research
Biosafety Level	2
Product Format	frozen
Type Strain	yes

Genome Sequenced Strain	Yes
Comments	Psychrophilic fungus. Pathogen causing bat white-nose syndrome. Genome sequencing strain (The Broad Institute, USA). Individuals that have contact with G. destructans should observe a personal quarantine and not enter bat congregation sites during active work and for 7 days after last contact with the fungus. Lab processes should be managed to avoid inadvertent contact with culture materials. Clothing and other materials brought into the laboratory should be segregated from those transported to any potential bat congregation sites.
Morphology	On SDA at 10C after 38 days, colonies white to grayish white, raised, dense velvety texture, slow growing, colony diameter after 38 days 1.8-2.3cm. Production of light to dark brown exudate. Reverse buff to tan. Hyphae guttulate, 2.25µm thick. Conidiophores with whorls of 2-6 verticils. Conidia formed singly and in short chains of 2-5, smooth, amygdaliform to allantoids with apical scar, containing oil droplets, 6-10.5µm X 3-4.5µm.

Medium	ATCC^® Medium 336: Potato dextrose agar (PDA) ATCC^® Medium 28: Emmons' modification of Sabouraud's agar
Growth Conditions	Temperature: 4°C to 10°C Atmosphere: Typical aerobic

Sequenced Data	>18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence. GAAACCGTTCCGTAGGTGAACCTGCGGAAGGATCATTACAGTAGTCGCCCGGGTTGCCGCAAGGCCTCCCGGGTAACCTACCACCCTTTGTTTATTACACTTTGTTGCTTTGGCAGGCCTGCCCTCGGGCTGCTGGCTCCGGCCGGCGAGCGCTTGCCAGAGGACTAAACTCTGTTTGTCTATACTGTCTGAGTACTATATAATAGTTAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTACAACCCTCAAGCTCAGCTTGGTATTGGGCCCCGCCGACCCGGCGGGCCCTAAAGTCAGTGGCGGTGCCGTCCGGCTCCGAGCGTAGTAATTCTTCTCGCTCCGGAGGTCCGGTCGTGTGCTTGCCAGCAACCCCCAATTTTTTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAA >D1D2 region of the 28S ribosomal RNA gene: AACCAACAGGGATTACCTCAGTAACGGCGAGTGAAGCGGTAACAGCTCAAATTTGAAATCTGGCCTCACGGTCCGAGTTGTAATTTGTAGAGGATGCTTCGAGCATGGTCTGGCCTAAGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTATGCGGCCAGGTGCCTACGCTCATGTGAAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCAAAATGGGTGGTAAATTTCATCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAGCGCTTGCAACCAGACTTGCGCGCGGCCGATCATCCGGTGTTCTCACCGGTGCACTCGGCCGTGCTCAGGCCAGCATCGGTTTTGGCGGCTGGATAAAGGCCCTAGGAATGTAGCTCCTCTCGGGGAGTGTTATAGTCTAGGGTGCAATGCAGCCTGCTGGGACCGAGGACCGCGCTTCGGCTAGGATGCTGGCGTAATGGTTGTAAGCGGCCCGTCTTG >18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence. GAAACCGTTCCGTAGGTGAACCTGCGGAAGGATCATTACAGTAGTCG CCCGGGTTGCCGCAAGGCCTCCCGGGTAACCTACCACCCTTTGTTTA TTACACTTTGTTGCTTTGGCAGGCCTGCCCTCGGGCTGCTGGCTCCG GCCGGCGAGCGCTTGCCAGAGGACTAAACTCTGTTTGTCTATACTGT CTGAGTACTATATAATAGTTAAAACTTTCAACAACGGATCTCTTGGT TCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAAT TGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCC CTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTACAACCCTCA AGCTCAGCTTGGTATTGGGCCCCGCCGACCCGGCGGGCCCTAAAGTC AGTGGCGGTGCCGTCCGGCTCCGAGCGTAGTAATTCTTCTCGCTCCG GAGGTCCGGTCGTGTGCTTGCCAGCAACCCCCAATTTTTTCAGGTTG ACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAA >D1D2 region of the 28S ribosomal RNA gene: AACCAACAGGGATTACCTCAGTAACGGCGAGTGAAGCGGTAACAGCT CAAATTTGAAATCTGGCCTCACGGTCCGAGTTGTAATTTGTAGAGGA TGCTTCGAGCATGGTCTGGCCTAAGTTCCTTGGAACAGGACGTCATA GAGGGTGAGAATCCCGTATGCGGCCAGGTGCCTACGCTCATGTGAAG CTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCAAAATGGGTG GTAAATTTCATCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCAC AAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAA CAGTACGTGAAATTGTTGAAAGGGAAGCGCTTGCAACCAGACTTGCG CGCGGCCGATCATCCGGTGTTCTCACCGGTGCACTCGGCCGTGCTCA GGCCAGCATCGGTTTTGGCGGCTGGATAAAGGCCCTAGGAATGTAGC TCCTCTCGGGGAGTGTTATAGTCTAGGGTGCAATGCAGCCTGCTGGG ACCGAGGACCGCGCTTCGGCTAGGATGCTGGCGTAATGGTTGTAAGC GGCCCGTCTTG
Morphology	On SDA at 10C after 38 days, colonies white to grayish white, raised, dense velvety texture, slow growing, colony diameter after 38 days 1.8-2.3cm. Production of light to dark brown exudate. Reverse buff to tan. Hyphae guttulate, 2.25µm thick. Conidiophores with whorls of 2-6 verticils. Conidia formed singly and in short chains of 2-5, smooth, amygdaliform to allantoids with apical scar, containing oil droplets, 6-10.5µm X 3-4.5µm.

Sequenced Data

>18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence.

GAAACCGTTCCGTAGGTGAACCTGCGGAAGGATCATTACAGTAGTCGCCCGGGTTGCCGCAAGGCCTCCCGGGTAACCTACCACCCTTTGTTTATTACACTTTGTTGCTTTGGCAGGCCTGCCCTCGGGCTGCTGGCTCCGGCCGGCGAGCGCTTGCCAGAGGACTAAACTCTGTTTGTCTATACTGTCTGAGTACTATATAATAGTTAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTACAACCCTCAAGCTCAGCTTGGTATTGGGCCCCGCCGACCCGGCGGGCCCTAAAGTCAGTGGCGGTGCCGTCCGGCTCCGAGCGTAGTAATTCTTCTCGCTCCGGAGGTCCGGTCGTGTGCTTGCCAGCAACCCCCAATTTTTTCAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAA

>D1D2 region of the 28S ribosomal RNA gene:

AACCAACAGGGATTACCTCAGTAACGGCGAGTGAAGCGGTAACAGCTCAAATTTGAAATCTGGCCTCACGGTCCGAGTTGTAATTTGTAGAGGATGCTTCGAGCATGGTCTGGCCTAAGTTCCTTGGAACAGGACGTCATAGAGGGTGAGAATCCCGTATGCGGCCAGGTGCCTACGCTCATGTGAAGCTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCAAAATGGGTGGTAAATTTCATCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAACAGTACGTGAAATTGTTGAAAGGGAAGCGCTTGCAACCAGACTTGCGCGCGGCCGATCATCCGGTGTTCTCACCGGTGCACTCGGCCGTGCTCAGGCCAGCATCGGTTTTGGCGGCTGGATAAAGGCCCTAGGAATGTAGCTCCTCTCGGGGAGTGTTATAGTCTAGGGTGCAATGCAGCCTGCTGGGACCGAGGACCGCGCTTCGGCTAGGATGCTGGCGTAATGGTTGTAAGCGGCCCGTCTTG

>18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial sequence. GAAACCGTTCCGTAGGTGAACCTGCGGAAGGATCATTACAGTAGTCG CCCGGGTTGCCGCAAGGCCTCCCGGGTAACCTACCACCCTTTGTTTA TTACACTTTGTTGCTTTGGCAGGCCTGCCCTCGGGCTGCTGGCTCCG GCCGGCGAGCGCTTGCCAGAGGACTAAACTCTGTTTGTCTATACTGT CTGAGTACTATATAATAGTTAAAACTTTCAACAACGGATCTCTTGGT TCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAAT TGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCC CTGGTATTCCGGGGGGCATGCCTGTCCGAGCGTCATTACAACCCTCA AGCTCAGCTTGGTATTGGGCCCCGCCGACCCGGCGGGCCCTAAAGTC AGTGGCGGTGCCGTCCGGCTCCGAGCGTAGTAATTCTTCTCGCTCCG GAGGTCCGGTCGTGTGCTTGCCAGCAACCCCCAATTTTTTCAGGTTG ACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAA >D1D2 region of the 28S ribosomal RNA gene: AACCAACAGGGATTACCTCAGTAACGGCGAGTGAAGCGGTAACAGCT CAAATTTGAAATCTGGCCTCACGGTCCGAGTTGTAATTTGTAGAGGA TGCTTCGAGCATGGTCTGGCCTAAGTTCCTTGGAACAGGACGTCATA GAGGGTGAGAATCCCGTATGCGGCCAGGTGCCTACGCTCATGTGAAG CTCCTTCGACGAGTCGAGTTGTTTGGGAATGCAGCTCAAAATGGGTG GTAAATTTCATCTAAAGCTAAATATTGGCCAGAGACCGATAGCGCAC AAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTTAAA CAGTACGTGAAATTGTTGAAAGGGAAGCGCTTGCAACCAGACTTGCG CGCGGCCGATCATCCGGTGTTCTCACCGGTGCACTCGGCCGTGCTCA GGCCAGCATCGGTTTTGGCGGCTGGATAAAGGCCCTAGGAATGTAGC TCCTCTCGGGGAGTGTTATAGTCTAGGGTGCAATGCAGCCTGCTGGG ACCGAGGACCGCGCTTCGGCTAGGATGCTGGCGTAATGGTTGTAAGC GGCCCGTCTTG

Morphology

On SDA at 10C after 38 days, colonies white to grayish white, raised, dense velvety texture, slow growing, colony diameter after 38 days 1.8-2.3cm. Production of light to dark brown exudate. Reverse buff to tan. Hyphae guttulate, 2.25µm thick. Conidiophores with whorls of 2-6 verticils. Conidia formed singly and in short chains of 2-5, smooth, amygdaliform to allantoids with apical scar, containing oil droplets, 6-10.5µm X 3-4.5µm.

Name of Depositor	DS Blehert
Special Collection	ATCC
Chain of Custody	ATCC <-- DS Blehert
Isolation	Wing skin of Myotis lucifugus, little brown bat, in Williams Hotel Mine, Ulster County, New York, USA. Isolation date: February 2008.
Year of Origin	2008
Cross References	Nucleotide (GenBank) : EU884921 Geomyces destructans isolate 20631-21 small subunit ribosomal RNA gene, partial sequence; internal transcribed spacer 1 and 5.8S ribosomal RNA gene, complete sequence; and internal transcribed spacer 2, partial sequence. Nucleotide (GenBank) : FJ231098 Geomyces destructans isolate 20631-21 18S small subunit ribosomal RNA gene, partial sequence. Complete Genome (GenBank) : AEFC00000000 Geomyces destructans 20631-21, whole genome shotgun sequencing project
References	Gargas A, et al. Geomyces destructans sp. nov. associated with bat white-nose syndrome. Mycotaxon 108: 147-154, 2009. Lorch JM, et al. Experimental infection of bats with Geomyces destructans causes white-nose syndrome. Nature 480: 376-378, 2011. PubMed: 22031324 Blehert DS, et al. Bat white-nose syndrome: an emerging fungal pathogen? Science 323(5911): 227, 2009. PubMed: 18974316 Chaturvedi V, et al. Morphological and molecular characterizations of psychrophilic fungus Geomyces destructans from New York bats with White Nose Syndrome (WNS). PLoS ONE 5(5): e10783. PubMed: 20520731

Permits	These permits may be required for shipping this product: Customer Acceptance of Responsibility, ATCC Form 62 required for distribution. Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation	Product Sheet Certificate of Analysis MSDS

Geomyces destructans Blehert et Gargas (ATCC® MYA-4855™)

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Geomyces destructans Blehert et Gargas (ATCC^® MYA-4855^™)