pTR-UF-11 plasmid in E. coli (ATCC® MBA-331)

Applications: SmaI cuts in both AAV ITRs and can serve as a diagnostic that the ITRs are intact.  / 

Designations pTR-UF-11 plasmid in E. coli
Biosafety Level 1
Vector Information
Cloning sites: NotI sites (nt 1922 and 2654), AccI (nt 2862 and 3961).
Transcription terminators: SV40 polyA, bovine growth hormone polyA.
Suggested host: E. coli SURE (Stratagene)
Applications
SmaI cuts in both AAV ITRs and can serve as a diagnostic that the ITRs are intact.
Comments
NOTE: this vector harbors AAV2 Inverted Terminal repeats (AAV ITRs)which can be unstable in bacteria. When culturing the bacteria containing AAV ITRs, do not culture for more than 16-18 hours at 37C because deletions may occur especially in stationary cultures.
A missing ITR can be tolerated in about 20% of a plasmid prep. SmaI cuts in both AAV ITRs and can serve as a diagnostic that the ITRs are intact. One or the other ITRs may be deleted (and so will the SmaI site) during culturing and cloning. If an ITR is deleted, then the SmaI digests will generate larger molecular weight bands.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
Related Products
ATCC VR-1616
Shipped frozen