60bca (ATCC® HB-247™)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with human CD14, inhibits the binding of LPS - LPS binding protein complexes to CD14 on human phagocytes and prevents the release of tumor necrosis factor alpha.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD14
Comments
Animals were immunized with cultured human peripheral blood monocytes.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with human CD14, inhibits the binding of LPS - LPS binding protein complexes to CD14 on human phagocytes and prevents the release of tumor necrosis factor alpha.
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • heat-inactivated gamma globulin free horse serum to a final concentration of 10%
    • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG1; kappa light chain
Name of Depositor RF Todd
References

Ashmun RA, et al. Expression of the human monocyte membrane antigen gp55 by murine fibroblasts after DNA-mediated gene transfer. Blood 69: 886-892, 1987. PubMed: 3545322

Wright SD, et al. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science 249: 1431-1433, 1990. PubMed: 1698311

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
References

Ashmun RA, et al. Expression of the human monocyte membrane antigen gp55 by murine fibroblasts after DNA-mediated gene transfer. Blood 69: 886-892, 1987. PubMed: 3545322

Wright SD, et al. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science 249: 1431-1433, 1990. PubMed: 1698311