26ic (ATCC® HB-246)

Organism: Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)  /  Cell Type: hybridoma: B lymphocyte  / 

Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1
Applications
The antibody reacts with a nonfunctional domain of human CD14.
The binding of 26ic does not inhibit CD14 mediated activities, and is useful for detecting CD14 expression by immunofluorescence and or immunocytochemical methods.
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against human CD14
Comments
Animals were immunized with cultured human peripheral blood monocytes.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with a nonfunctional domain of human CD14.
The binding of 26ic does not inhibit CD14 mediated activities, and is useful for detecting CD14 expression by immunofluorescence and or immunocytochemical methods.
Complete Growth Medium The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • heat-inactivated gamma globulin free horse serum to a final concentration of 10%
  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere

Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Isotype IgG2b; kappa light chain
Name of Depositor RF Todd
References

Todd RF, et al. Structural analysis of differentiation antigens Mo1 and Mo2 on human monocytes. Hybridoma 1: 329-337, 1982. PubMed: 6208133

Wright SD, et al. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science 249: 1431-1433, 1990. PubMed: 1698311

Basic Documentation
References

Todd RF, et al. Structural analysis of differentiation antigens Mo1 and Mo2 on human monocytes. Hybridoma 1: 329-337, 1982. PubMed: 6208133

Wright SD, et al. CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science 249: 1431-1433, 1990. PubMed: 1698311