JHC7 (ATCC® CRL-3267)

Organism: Homo sapiens, human  /  Tissue: sacral bone  /  Disease: tumor, chordoma

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Organism Homo sapiens, human
Tissue sacral bone
Product Format frozen
Morphology epithelial-like
Culture Properties adherent
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease tumor, chordoma
Age 61 years old
Gender female
Ethnicity Caucasian
Applications

May serve as a platform for the evaluation of several chemotherapeutic strategies to treat chordoma as it accurately recapitulates chordoma. The Brachyury expression may facilitate understanding of its role in other epithelial-derived cancers.

The Chordoma Foundation may be able to offer financial assistance for the purchase of this cell line. Please contact cells@chordoma.org for more information.

Storage Conditions liquid nitrogen vapor phase
Images Cell Micrograph of JHC7, ATCC CRL-3267
Derivation

Fresh surgical chordoma tissue was minced in 0.25% trypsin then placed on shaker at 37°C for 30 minutes. The tissue was then triturated and passed through 40 µm nylon mesh. Single cells were plated and the culture expanded. (source: PubMed: 21699479)

Tumorigenic Yes, in SCID mice (source: PubMed: 21699479)
Comments

CRL-3267 is a primary chordoma cell line from a sacral tumor that expresses Brachyury. This cell line’s classical phenotypic features, such as characteristic physaliferous cells and stable expression of Brachyury, are maintained through serial passaging.

Expresses embryonic transcription factor Brachyury. This cell line was accessioned with the support of the Chordoma Foundation, a non-profit organization working to improve the lives of chordoma patients by accelerating research to develop effective treatments for the chordoma disease.

Complete Growth Medium The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing

Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium. Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) (ATCC 30-2200) or 0.25% (w/v) Trypsin - 0.53 mM EDTA (ATCC 30-2101) solution to remove all traces of serum which contains trypsin inhibitor.
  2. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
  3. Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Transfer cell suspension to a centrifuge tube and spin at approximately 125 x g for 5 to 10 minutes.
  6. Discard supernatant. Resuspend the cell pellet in fresh growth medium.
  7. Add appropriate aliquots of the cell suspension to new culture vessels
  8. Incubate cultures at 37°C.

 

Subcultivation Ratio: 1:2 to 1:3 is recommended.

Medium Renewal: 2 to 3 times a week

Cryopreservation Freeze Medium: complete growth medium, 90%; DMSO, 10%
Storage Temperature: liquid nitrogen vapor phase
Culture Conditions Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 11
D5S818: 13
D7S820: 7, 10
TH01: 6, 8
TPOX: 10, 11
vWA: 17
Passage Number 59
Population Doubling Time Approximately 5 days (source: PubMed: 21699479)
Name of Depositor A Quinones-Hinojosa, Johns Hopkins University
Year of Origin 2010
References

Hsu W, et.al. Generation of chordoma cell line JHC7 and the identification of Brachyury as a novel molecular target. J. Neurosurg. 115(4): 760-769, 2011. PubMed: 21699479

Notice: Necessary PermitsPermits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Other Documentation
References

Hsu W, et.al. Generation of chordoma cell line JHC7 and the identification of Brachyury as a novel molecular target. J. Neurosurg. 115(4): 760-769, 2011. PubMed: 21699479