JVM-13 (ATCC® CRL-3003™)

Organism: Homo sapiens, human  /  Tissue: peripheral blood, B-prolymphocytic leukemia (B-PLL)  /  Cell Type: lymphoblast; immortalized with Epstein-Barr virus (EBV); Epstein-Barr virus (EBV) transforme

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Organism Homo sapiens, human
Tissue
peripheral blood, B-prolymphocytic leukemia (B-PLL)
Cell Type lymphoblast; immortalized with Epstein-Barr virus (EBV); Epstein-Barr virus (EBV) transforme
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 2
Disease B-prolymphocytic leukemia (B-PLL)
Age late 60's
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Karyotype Modal chromosome number: 47 This is a pseudodiploid human cell line of male origin with a modal chromosome number of 47. Most of the cells contain the derivative chromosomes: add(2q), t(8;13)(p12;q13) and t(8;?)(p12;?). Overall, the karyology was consistent with other published data [PubMed: 3262465].
Images
Clinical Data
Leukemic cells from a patient with B-prolymphocytic leukemia were immortalized in vitro with Epstein-Barr virus (EBV) in the presence of phorbol-ester, TPA.
male
Caucasian
late 60's
Antigen Expression
CD3-, CD5-, CD10-, CD19+, CD20+, CD23+, FMC7+ (verified at ATCC)
Genes Expressed
CD3-, CD5-, CD10-, CD19+, CD20+, CD23+, FMC7+ (verified at ATCC)
Comments
Leukemic cells from a patient with B-prolymphocytic leukemia were immortalized in vitro with Epstein-Barr virus (EBV) in the presence of phorbol-ester, TPA.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10(4) to 4 x 10(4) viable cells/ml. Do not allow the cell concentration to exceed 1 X 10(6) cells/ml.
Cryopreservation
70% growth medium; 20% FBS; 10% DMSO
Culture Conditions
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
STR Profile
Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 9,11
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 6,7
TPOX: 10,11
vWA: 16,18
Population Doubling Time about 58 hours
Name of Depositor J.V.Melo
References

Melo JV, et al. Two new cell lines from B-prolymphocytic leukaemia: characterization by morphology, immunological markers, karyotype and Ig gene rearrangement. Int. J. Cancer 38: 531-538, 1986. PubMed: 3093393

Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365

Permits Notice: Necessary Permits

These permits may be required for shipping this product:

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.
Basic Documentation
Product Sheet
Product Sheet
Certificate of Analysis
Certificate of Analysis
MSDS
MSDS
Other Documentation
Other Document
Cell Micrograph Other Document
Characterization Data
References

Melo JV, et al. Two new cell lines from B-prolymphocytic leukaemia: characterization by morphology, immunological markers, karyotype and Ig gene rearrangement. Int. J. Cancer 38: 531-538, 1986. PubMed: 3093393

Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365